Sirtuins are part of a gene family of NAD-dependent deacylases that act on histone and non-histone proteins and control a variety of activities in all living organisms. Their roles are mainly related to energy metabolism and include lifetime regulation, DNA repair, stress resistance, and proliferation. A large amount of knowledge concerning animal sirtuins is available, but data about their plant counterparts are scarce. Plants possess few sirtuins that have, like in animals, a recognized role in stress defense and metabolism regulation. However, engagement in proliferation control, which has been demonstrated for mammalian sirtuins, has not been reported for plant sirtuins so far. In this work, srt1 and srt2 Arabidopsis mutant seedlings have been used to evaluate in vivo the role of sirtuins in cell proliferation and regulation of glutamate dehydrogenase, an enzyme demonstrated to be involved in the control of cell cycle in SIRT4-defective human cells. Moreover, bioinformatic analyses have been performed to elucidate sequence, structure, and function relationships between Arabidopsis sirtuins and between each of them and the closest mammalian homolog. We found that cell proliferation and GDH activity are higher in mutant seedlings, suggesting that both sirtuins exert a physiological inhibitory role in these processes. In addition, mutant seedlings show plant growth and root system improvement, in line with metabolic data. Our data also indicate that utilization of an easy to manipulate organism, such as Arabidopsis plant, can help to shed light on the molecular mechanisms underlying the function of genes present in interkingdom species.

Arabidopsis thaliana sirtuins control proliferation and glutamate dehydrogenase activity / Bruscalupi, Giovannella; Di Micco, Patrizio; Maria Failla, Cristina; Pascarella, Gianmarco; Morea, Veronica; Saliola, Michele; De Paolis, Angelo; Venditti, Sabrina Donatella Sonia; Luisa Mauro, Maria. - In: PLANT PHYSIOLOGY AND BIOCHEMISTRY. - ISSN 0981-9428. - 194:(2023), pp. 236-245. [10.1016/j.plaphy.2022.11.007]

Arabidopsis thaliana sirtuins control proliferation and glutamate dehydrogenase activity

Giovannella Bruscalupi
Primo
Investigation
;
Gianmarco Pascarella
Software
;
Michele Saliola
Membro del Collaboration Group
;
Sabrina Venditti
Penultimo
Membro del Collaboration Group
;
2023

Abstract

Sirtuins are part of a gene family of NAD-dependent deacylases that act on histone and non-histone proteins and control a variety of activities in all living organisms. Their roles are mainly related to energy metabolism and include lifetime regulation, DNA repair, stress resistance, and proliferation. A large amount of knowledge concerning animal sirtuins is available, but data about their plant counterparts are scarce. Plants possess few sirtuins that have, like in animals, a recognized role in stress defense and metabolism regulation. However, engagement in proliferation control, which has been demonstrated for mammalian sirtuins, has not been reported for plant sirtuins so far. In this work, srt1 and srt2 Arabidopsis mutant seedlings have been used to evaluate in vivo the role of sirtuins in cell proliferation and regulation of glutamate dehydrogenase, an enzyme demonstrated to be involved in the control of cell cycle in SIRT4-defective human cells. Moreover, bioinformatic analyses have been performed to elucidate sequence, structure, and function relationships between Arabidopsis sirtuins and between each of them and the closest mammalian homolog. We found that cell proliferation and GDH activity are higher in mutant seedlings, suggesting that both sirtuins exert a physiological inhibitory role in these processes. In addition, mutant seedlings show plant growth and root system improvement, in line with metabolic data. Our data also indicate that utilization of an easy to manipulate organism, such as Arabidopsis plant, can help to shed light on the molecular mechanisms underlying the function of genes present in interkingdom species.
2023
Sirtuins; Arabidopsis thaliana; Proliferation; Glutamate dehydrogenase; Sirtuin 3D models
01 Pubblicazione su rivista::01a Articolo in rivista
Arabidopsis thaliana sirtuins control proliferation and glutamate dehydrogenase activity / Bruscalupi, Giovannella; Di Micco, Patrizio; Maria Failla, Cristina; Pascarella, Gianmarco; Morea, Veronica; Saliola, Michele; De Paolis, Angelo; Venditti, Sabrina Donatella Sonia; Luisa Mauro, Maria. - In: PLANT PHYSIOLOGY AND BIOCHEMISTRY. - ISSN 0981-9428. - 194:(2023), pp. 236-245. [10.1016/j.plaphy.2022.11.007]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1673091
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