This paper presents a custom, low-cost electronic system specifically designed for rapid and quantitative detection of the malaria parasite in a blood sample. The system exploits the paramagnetic properties of malaria-infected red blood cells (iRBCs) for their magnetophoretic capture on the surface of a silicon chip. A lattice of nickel magnetic micro-concentrators embedded in a silicon substrate concentrates the iRBCs above coplanar gold microelectrodes separated by 3 μm for their detection through an impedance measurement. The sensor is designed for a differential operation to remove the large contribution given by the blood sample. The electronic readout automatically balances the sensor before each experiment and reaches a resolution of 15 ppm in the impedance measurement at 1 MHz allowing a limit of detection of 40 parasite/μl with a capture time of 10 minutes. For better reliability of the results, four sensors are acquired during the same experiment. We demonstrate that the realized platform can also detect a single infected cell in real experimental conditions, measuring human blood infected by Plasmodium falciparum malaria specie.

Impedance-based rapid diagnostic tool for single malaria parasite detection / Giacometti, M; Pravettoni, T; Barsotti, J; Milesi, F; Figares, Co; Maspero, F; Coppadoro, Lp; Benevento, G; Ciardo, M; Alano, P; Fiore, Gb; Bertacco, R; Ferrari, G. - In: IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS. - ISSN 1932-4545. - 16:6(2022), pp. 1325-1336. [10.1109/tbcas.2022.3215586]

Impedance-based rapid diagnostic tool for single malaria parasite detection

Benevento G;Ciardo M;Alano P;
2022

Abstract

This paper presents a custom, low-cost electronic system specifically designed for rapid and quantitative detection of the malaria parasite in a blood sample. The system exploits the paramagnetic properties of malaria-infected red blood cells (iRBCs) for their magnetophoretic capture on the surface of a silicon chip. A lattice of nickel magnetic micro-concentrators embedded in a silicon substrate concentrates the iRBCs above coplanar gold microelectrodes separated by 3 μm for their detection through an impedance measurement. The sensor is designed for a differential operation to remove the large contribution given by the blood sample. The electronic readout automatically balances the sensor before each experiment and reaches a resolution of 15 ppm in the impedance measurement at 1 MHz allowing a limit of detection of 40 parasite/μl with a capture time of 10 minutes. For better reliability of the results, four sensors are acquired during the same experiment. We demonstrate that the realized platform can also detect a single infected cell in real experimental conditions, measuring human blood infected by Plasmodium falciparum malaria specie.
2022
malaria; parasite; electronic sistem; detection
01 Pubblicazione su rivista::01a Articolo in rivista
Impedance-based rapid diagnostic tool for single malaria parasite detection / Giacometti, M; Pravettoni, T; Barsotti, J; Milesi, F; Figares, Co; Maspero, F; Coppadoro, Lp; Benevento, G; Ciardo, M; Alano, P; Fiore, Gb; Bertacco, R; Ferrari, G. - In: IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS. - ISSN 1932-4545. - 16:6(2022), pp. 1325-1336. [10.1109/tbcas.2022.3215586]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1673050
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