Objectives: To evaluate whether the increase of temperature can influence the environmental endurance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: Virus was inoculated on a plastic surface and harvested at predefined time-points in parallel at 20°C–25°C (room temperature; RT) and at 28°C (June temperature; JT). Samples were tested by TCID50 titres on Vero cells. Results: Our results confirm that fomite transmission of the emerging SARS-CoV-2 is possible: the virus reserved its ability to infect cells for up to 84 hours at both RT and JT on a plastic surface, with TCID50 viral titres of 0.67 and 0.25 log10, respectively. At RT, an important reduction in the viral titre, from 4 log10 to 3 log10 TCID50, was observed during the first 24–36 hours. At JT, the same decay was observed more rapidly (between 8 and 12 hours), The rate of viral inactivation by D-value was 24.74 hours at RT and 12.21 hours at JT. Conclusions: This remarkable difference between the two temperatures suggests that virus vitality can be influenced by the environmental temperature and that the hot season could reduce the probability of COVID-19 transmission.

SARS-CoV-2 infection. The environmental endurance of the virus can be influenced by the increase of temperature / Magurano, Fabio; Baggieri, Melissa; Marchi, Antonella; Rezza, Giovanni; Nicoletti, Loredana; Stefanelli, Paola; Benedetti, Eleonora; Fiore, Stefano; Fabiani, Concetta; Kojouri, Maedeh; Bucci, Paola; D’Ugo, Emilio; Gioacchini, Silvia. - In: CLINICAL MICROBIOLOGY AND INFECTION. - ISSN 1198-743X. - 27:2(2021), pp. 289.e5-289.e7. [10.1016/j.cmi.2020.10.034]

SARS-CoV-2 infection. The environmental endurance of the virus can be influenced by the increase of temperature

Kojouri, Maedeh;Gioacchini, Silvia
2021

Abstract

Objectives: To evaluate whether the increase of temperature can influence the environmental endurance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: Virus was inoculated on a plastic surface and harvested at predefined time-points in parallel at 20°C–25°C (room temperature; RT) and at 28°C (June temperature; JT). Samples were tested by TCID50 titres on Vero cells. Results: Our results confirm that fomite transmission of the emerging SARS-CoV-2 is possible: the virus reserved its ability to infect cells for up to 84 hours at both RT and JT on a plastic surface, with TCID50 viral titres of 0.67 and 0.25 log10, respectively. At RT, an important reduction in the viral titre, from 4 log10 to 3 log10 TCID50, was observed during the first 24–36 hours. At JT, the same decay was observed more rapidly (between 8 and 12 hours), The rate of viral inactivation by D-value was 24.74 hours at RT and 12.21 hours at JT. Conclusions: This remarkable difference between the two temperatures suggests that virus vitality can be influenced by the environmental temperature and that the hot season could reduce the probability of COVID-19 transmission.
2021
coronavirus disease 2019; infectious disease; outbreak; severe acute respiratory syndrome coronavirus 2; temperature; viral spread; viral stability; animals; covid-19; chlorocebus aethiops; fomites; humans; microbial viability; Ssars-cov-2; temperature; vero cells; viral load; virus inactivation; environmental microbiology
01 Pubblicazione su rivista::01a Articolo in rivista
SARS-CoV-2 infection. The environmental endurance of the virus can be influenced by the increase of temperature / Magurano, Fabio; Baggieri, Melissa; Marchi, Antonella; Rezza, Giovanni; Nicoletti, Loredana; Stefanelli, Paola; Benedetti, Eleonora; Fiore, Stefano; Fabiani, Concetta; Kojouri, Maedeh; Bucci, Paola; D’Ugo, Emilio; Gioacchini, Silvia. - In: CLINICAL MICROBIOLOGY AND INFECTION. - ISSN 1198-743X. - 27:2(2021), pp. 289.e5-289.e7. [10.1016/j.cmi.2020.10.034]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1672773
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