INTRODUCTION: Aedes Invasive mosquitoes (aIm) play an important role in public health, as vectors of pathogens and a source of nuisance for humans and animals. european and national guidelines for the surveillance of aIm suggest several methods for the monitoring of these species. In this study, we compared the performance of three traps for monitoring aIm. MATERIALS AND METHODS: we compared two types of ovitraps containing 0.25 l and 1 l of water respectively, and a Sticky Gravid Trap (SGT). we named the two ovitraps, Standard ovitrap (Sov) and aIm-CoST ovitrap (Cov), since the latter has been proposed by the aIm-CoST action Team. we selected five sites in five cities in veneto and friuli venezia Giulia regions: Sommacampagna (vr), Padova (Pd), occhiobello (ro), belluno (bl), and Tolmezzo (ud); the three traps were placed in each site. every two weeks, we collected the samples (eggs and adult mosquitoes) and rotated the traps, according to a 3x3 latin square. adult mosquitoes and eggs were counted and identified in IzSve laboratory (eggs were hatched, first and fourth instar larvae were identified). RESULTS AND CONCLUSIONS: from June 2020 to october 2020, we collected 416 samples by ovitraps (207 Sov and 209 Cov) and 218 by SGT. we observed no significant difference between the mean number of eggs collected by the two ovitraps (Sov=138.64, Cov=131.36, p>0.05). The mean number of aIm females collected by SGT was 6.10 per trap. we observed no correlation between adults and eggs number (r2 Sov=0.32; r2 Cov=0.24). In three sites (Sommacampagna, Padova, occhiobello) we collected only Aedes albopictus, as eggs and adults. In belluno, Ae. albopictus was the most abundant species (78.8% of collected eggs and 89.9% of adults), followed by Ae. koreicus and Ae. japonicus. In Tolmezzo, Ae japonicus was the most abundant species (79.8% of collected eggs and 79.7% of adults), followed by Ae. geniculatus and Ae. albopictus. In conclusion, both types of ovitraps are good in collecting Aedes eggs. entomologists prefer ovitraps containing a larger volume of water, because they are unlikely to dry out during the summer season, avoiding the need to increase the frequency of sample collection. In our study, Sov were not affected by drought, probably because they were placed in a rainy area. SGT are a good method for aIm collection. unfortunately, the engorged females collected by SGT were not further analyzed (e.g. screening of pathogens or hosts preference) because the specimens were easily damaged during handling. The method could be improved by studying a less aggressive sticky sheet, but equally effective in mosquitoes collection.
Comparison of two types of ovitraps and sticky gravid trap for monitoring aedes invasive mosquitoes / Gradoni, F.; Carlin, S.; Bertola, M.; Visentin, P.; Dal Pont, M.; Adami, S.; Di Domenico, D.; Zuliani, D.; Manzi, S.; Michelutti, A.; Montarsi, F.. - (2022), p. 235. (Intervento presentato al convegno XXXII congresso della Società Italiana di Parassitologia tenutosi a Napoli).
Comparison of two types of ovitraps and sticky gravid trap for monitoring aedes invasive mosquitoes
Manzi S.;Montarsi F.
2022
Abstract
INTRODUCTION: Aedes Invasive mosquitoes (aIm) play an important role in public health, as vectors of pathogens and a source of nuisance for humans and animals. european and national guidelines for the surveillance of aIm suggest several methods for the monitoring of these species. In this study, we compared the performance of three traps for monitoring aIm. MATERIALS AND METHODS: we compared two types of ovitraps containing 0.25 l and 1 l of water respectively, and a Sticky Gravid Trap (SGT). we named the two ovitraps, Standard ovitrap (Sov) and aIm-CoST ovitrap (Cov), since the latter has been proposed by the aIm-CoST action Team. we selected five sites in five cities in veneto and friuli venezia Giulia regions: Sommacampagna (vr), Padova (Pd), occhiobello (ro), belluno (bl), and Tolmezzo (ud); the three traps were placed in each site. every two weeks, we collected the samples (eggs and adult mosquitoes) and rotated the traps, according to a 3x3 latin square. adult mosquitoes and eggs were counted and identified in IzSve laboratory (eggs were hatched, first and fourth instar larvae were identified). RESULTS AND CONCLUSIONS: from June 2020 to october 2020, we collected 416 samples by ovitraps (207 Sov and 209 Cov) and 218 by SGT. we observed no significant difference between the mean number of eggs collected by the two ovitraps (Sov=138.64, Cov=131.36, p>0.05). The mean number of aIm females collected by SGT was 6.10 per trap. we observed no correlation between adults and eggs number (r2 Sov=0.32; r2 Cov=0.24). In three sites (Sommacampagna, Padova, occhiobello) we collected only Aedes albopictus, as eggs and adults. In belluno, Ae. albopictus was the most abundant species (78.8% of collected eggs and 89.9% of adults), followed by Ae. koreicus and Ae. japonicus. In Tolmezzo, Ae japonicus was the most abundant species (79.8% of collected eggs and 79.7% of adults), followed by Ae. geniculatus and Ae. albopictus. In conclusion, both types of ovitraps are good in collecting Aedes eggs. entomologists prefer ovitraps containing a larger volume of water, because they are unlikely to dry out during the summer season, avoiding the need to increase the frequency of sample collection. In our study, Sov were not affected by drought, probably because they were placed in a rainy area. SGT are a good method for aIm collection. unfortunately, the engorged females collected by SGT were not further analyzed (e.g. screening of pathogens or hosts preference) because the specimens were easily damaged during handling. The method could be improved by studying a less aggressive sticky sheet, but equally effective in mosquitoes collection.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
