A possible role of reduced cytochrome c oxidase in the metabolism of nitric oxide (NO) has been examined with amperometric and stopped-flow photometric techniques. Reduced purified cytochrome c oxidase and mitochondria showed no catalytic reaction with NO under anaerobic conditions within more than 30 minutes. Only fast binding of NO to the reduced enzyme in a 1:1 stoichiometric ratio was observed. The NO binding rate was strongly decreased in the presence of 1 mM cyanide. These data indicate that, contrary to previous proposals, cytochrome c oxidase in the absence of oxygen does not contribute to physiological NO metabolism I.F.2.78

Cytochrome c oxidase does not catalyze the anaerobic reduction of NO / Gottfried, Stubauer; Giuffre', Alessandro; Brunori, Maurizio; Sarti, Paolo. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 245:2(1998), pp. 459-465. [10.1006/bbrc.1998.8457]

Cytochrome c oxidase does not catalyze the anaerobic reduction of NO

GIUFFRE', ALESSANDRO;BRUNORI, Maurizio;SARTI, Paolo
1998

Abstract

A possible role of reduced cytochrome c oxidase in the metabolism of nitric oxide (NO) has been examined with amperometric and stopped-flow photometric techniques. Reduced purified cytochrome c oxidase and mitochondria showed no catalytic reaction with NO under anaerobic conditions within more than 30 minutes. Only fast binding of NO to the reduced enzyme in a 1:1 stoichiometric ratio was observed. The NO binding rate was strongly decreased in the presence of 1 mM cyanide. These data indicate that, contrary to previous proposals, cytochrome c oxidase in the absence of oxygen does not contribute to physiological NO metabolism I.F.2.78
1998
cytochrome c oxidase; metabolism; nitric oxide
01 Pubblicazione su rivista::01a Articolo in rivista
Cytochrome c oxidase does not catalyze the anaerobic reduction of NO / Gottfried, Stubauer; Giuffre', Alessandro; Brunori, Maurizio; Sarti, Paolo. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 245:2(1998), pp. 459-465. [10.1006/bbrc.1998.8457]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/16701
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