Microglia reactivity entails a large-scale remodeling of cellular geometry, but the behavior of the microtubule cytoskeleton during these changes remains unexplored. Here we show that activated microglia provide an example of microtubule reorganization from a non-centrosomal array of parallel and stable microtubules to a radial array of more dynamic microtubules. While in the homeostatic state, microglia nucleate microtubules at Golgi outposts, and activating signaling induces recruitment of nucleating material nearby the centrosome, a process inhibited by microtubule stabilization. Our results demonstrate that a hallmark of microglia reactivity is a striking remodeling of the microtubule cytoskeleton and suggest that while pericentrosomal microtubule nucleation may serve as a distinct marker of microglia activation, inhibition of microtubule dynamics may provide a different strategy to reduce microglia reactivity in inflammatory disease.

Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays / Rosito, Maria; Sanchini, Caterina; Gosti, Giorgio; Moreno, Manuela; De Panfilis, Simone; Giubettini, Maria; Debellis, Doriana; Catalano, Federico; Peruzzi, Giovanna; Marotta, Roberto; Indrieri, Alessia; De Leonibus, Elvira; De Stefano, Maria Egle; Ragozzino, Davide; Ruocco, Giancarlo; Di Angelantonio, Silvia; Bartolini, Francesca. - In: CELL REPORTS. - ISSN 2211-1247. - 42:2(2023). [10.1016/j.celrep.2023.112104]

Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays

Rosito, Maria
Co-primo
;
Sanchini, Caterina
Co-primo
;
Moreno, Manuela;De Stefano, Maria Egle;Ragozzino, Davide;Ruocco, Giancarlo;Di Angelantonio, Silvia
Ultimo
;
2023

Abstract

Microglia reactivity entails a large-scale remodeling of cellular geometry, but the behavior of the microtubule cytoskeleton during these changes remains unexplored. Here we show that activated microglia provide an example of microtubule reorganization from a non-centrosomal array of parallel and stable microtubules to a radial array of more dynamic microtubules. While in the homeostatic state, microglia nucleate microtubules at Golgi outposts, and activating signaling induces recruitment of nucleating material nearby the centrosome, a process inhibited by microtubule stabilization. Our results demonstrate that a hallmark of microglia reactivity is a striking remodeling of the microtubule cytoskeleton and suggest that while pericentrosomal microtubule nucleation may serve as a distinct marker of microglia activation, inhibition of microtubule dynamics may provide a different strategy to reduce microglia reactivity in inflammatory disease.
2023
activated microglia; alternatively activated microglia; microtubule; centrosome; γ-tubulin; MTOC; Golgi outposts; retina; inflammation; reactive microglia
01 Pubblicazione su rivista::01a Articolo in rivista
Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays / Rosito, Maria; Sanchini, Caterina; Gosti, Giorgio; Moreno, Manuela; De Panfilis, Simone; Giubettini, Maria; Debellis, Doriana; Catalano, Federico; Peruzzi, Giovanna; Marotta, Roberto; Indrieri, Alessia; De Leonibus, Elvira; De Stefano, Maria Egle; Ragozzino, Davide; Ruocco, Giancarlo; Di Angelantonio, Silvia; Bartolini, Francesca. - In: CELL REPORTS. - ISSN 2211-1247. - 42:2(2023). [10.1016/j.celrep.2023.112104]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1669493
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