Spent coffee grounds were treated to obtain suitable carriers for both physical and covalent immobilization of Candida rugosa lipase. In the first case, physical adsorption was directly performed after water/organic solvent extraction of the interferents from the support, while in the second case the delignification and the activation of the support with glutaraldehyde or KIO4 were necessary. The support materials obtained were characterized by Fourier transform attenuated total reflection IR, elemental analysis, and scanning electron microscope. Each immobilization method was investigated to obtain the best insoluble biocatalyst immobilized activity, efficiency, and recovery. Emphasis was given to the study of the water/organic solvent ratio in the immobilization mixture as it has proven to be a decisive parameter. The optimized immobilized biocatalyst obtained by adsorption mechanism showed up to 33 U∙g-1 of immobilized enzyme activity, and a higher pH, thermal, and storage stability than the free lipase. This solid biocatalyst also preserved 100 % of its activity after four recycles in p-nitrophenyl palmitate hydrolysis in hexane medium and permitted an almost 60 % conversion of milk fats in fatty acids after 18 h of reaction, maintaining this value for three reuses.

Immobilization of lipase on spent coffee grounds by physical and covalent methods: A comparison study / Girelli, Anna Maria; Chiappini, Viviana; Amadoro, Paolo. - In: BIOCHEMICAL ENGINEERING JOURNAL. - ISSN 1369-703X. - 192:(2023), pp. 1-12. [10.1016/j.bej.2023.108827]

Immobilization of lipase on spent coffee grounds by physical and covalent methods: A comparison study

Anna Maria, Girelli
Primo
;
Viviana, Chiappini
Secondo
Formal Analysis
;
Paolo, Amadoro
Membro del Collaboration Group
2023

Abstract

Spent coffee grounds were treated to obtain suitable carriers for both physical and covalent immobilization of Candida rugosa lipase. In the first case, physical adsorption was directly performed after water/organic solvent extraction of the interferents from the support, while in the second case the delignification and the activation of the support with glutaraldehyde or KIO4 were necessary. The support materials obtained were characterized by Fourier transform attenuated total reflection IR, elemental analysis, and scanning electron microscope. Each immobilization method was investigated to obtain the best insoluble biocatalyst immobilized activity, efficiency, and recovery. Emphasis was given to the study of the water/organic solvent ratio in the immobilization mixture as it has proven to be a decisive parameter. The optimized immobilized biocatalyst obtained by adsorption mechanism showed up to 33 U∙g-1 of immobilized enzyme activity, and a higher pH, thermal, and storage stability than the free lipase. This solid biocatalyst also preserved 100 % of its activity after four recycles in p-nitrophenyl palmitate hydrolysis in hexane medium and permitted an almost 60 % conversion of milk fats in fatty acids after 18 h of reaction, maintaining this value for three reuses.
2023
spent coffee grounds; lignocellulosic material; wastes recycling; lipase adsorption immobilization; milk fats hydrolysis
01 Pubblicazione su rivista::01a Articolo in rivista
Immobilization of lipase on spent coffee grounds by physical and covalent methods: A comparison study / Girelli, Anna Maria; Chiappini, Viviana; Amadoro, Paolo. - In: BIOCHEMICAL ENGINEERING JOURNAL. - ISSN 1369-703X. - 192:(2023), pp. 1-12. [10.1016/j.bej.2023.108827]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1668263
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