Over the past quarter-century, environmental DNA (eDNA) has been ascendant as a tool to detect, measure and monitor biodiversity (species and communities), as a means of elucidating biological interaction networks, and as a window into understanding past patterns of biodiversity. However, only recently has the potential of eDNA been realized in the botanical world. Here we synthesize the state of eDNA applications in botanical systems with emphases on aquatic, ancient, contemporary sediment, and airborne systems, with focus on both single-species approaches and multi-species community metabarcoding. Further, we identify how abiotic and biotic factors, taxonomic resolution, primer choice, spatiotemporal scales, and relative abundance influence the utilization and interpretation of airborne eDNA results. Lastly, we explore several areas and opportunities for further development of eDNA in plants, advancing our knowledge and understanding of the efficacy, utility, and cost-effectiveness, and ultimately facilitating increased adoption of eDNA analyses in botanical systems.

Environmental DNA as an emerging tool in botanical research / Johnson, Mark D.; Freeland, Joanna R.; Parducci, Laura; Evans, Darren M.; Meyer, Rachel S.; Molano-Flores, Brenda; Davis, Mark A.. - In: AMERICAN JOURNAL OF BOTANY. - ISSN 1537-2197. - (2023). [10.1002/ajb2.16120]

Environmental DNA as an emerging tool in botanical research

Laura Parducci
Writing – Review & Editing
;
2023

Abstract

Over the past quarter-century, environmental DNA (eDNA) has been ascendant as a tool to detect, measure and monitor biodiversity (species and communities), as a means of elucidating biological interaction networks, and as a window into understanding past patterns of biodiversity. However, only recently has the potential of eDNA been realized in the botanical world. Here we synthesize the state of eDNA applications in botanical systems with emphases on aquatic, ancient, contemporary sediment, and airborne systems, with focus on both single-species approaches and multi-species community metabarcoding. Further, we identify how abiotic and biotic factors, taxonomic resolution, primer choice, spatiotemporal scales, and relative abundance influence the utilization and interpretation of airborne eDNA results. Lastly, we explore several areas and opportunities for further development of eDNA in plants, advancing our knowledge and understanding of the efficacy, utility, and cost-effectiveness, and ultimately facilitating increased adoption of eDNA analyses in botanical systems.
2023
environmental DNA; plant DNA; biodiversity; botanical systems; sedimentary DNA; airborne DNA; pollen DNA; metabarcoding
01 Pubblicazione su rivista::01a Articolo in rivista
Environmental DNA as an emerging tool in botanical research / Johnson, Mark D.; Freeland, Joanna R.; Parducci, Laura; Evans, Darren M.; Meyer, Rachel S.; Molano-Flores, Brenda; Davis, Mark A.. - In: AMERICAN JOURNAL OF BOTANY. - ISSN 1537-2197. - (2023). [10.1002/ajb2.16120]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1667605
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