The production of vaccines of biological origin presents a tremendous challenge for re-searchers. In this context, animal cell cultures are an excellent alternative for the isolation and production of biologicals against several viruses, since they have an affinity with viruses and a great capacity for their replicability. Different variables have been studied to know the system’s ideal parameters, allowing it to obtain profitable and competitive products. Consequently, this work fo-cuses its efforts on evaluating an alternative for producing an anti‐influenza biological from MDCK cells using SuperPro Designer v8.0 software. The process uses the DMEN culture medium supple-mented with nutrients as raw material for cell development; the MDCK cells were obtained from a potential scale‐up with a final working volume of 500 L, four days of residence time, inoculum volume of 10%, and continuous working mode with up to a total of 7400 h/Yr of work. The scheme has the necessary equipment for the vaccine’s production, infection, and manufacture with yields of up to 416,698 units/h. In addition, it was estimated to be economically viable to produce recom-binant vaccines with competitive prices of up to 0.31 USD/unit.

A simulation analysis of an influenza vaccine production plant in areas of high humanitarian flow. A preliminary study for the region of norte de santander (colombia) / Contreras-ropero, J. E.; Ruiz-roa, S. L.; Garcia-martinez, J. B.; Urbina-suarez, N. A.; Lopez-barrera, G. L.; Barajas-solano, A. F.; Zuorro, A.. - In: APPLIED SCIENCES. - ISSN 2076-3417. - 12:1(2022). [10.3390/app12010183]

A simulation analysis of an influenza vaccine production plant in areas of high humanitarian flow. A preliminary study for the region of norte de santander (colombia)

Zuorro A.
2022

Abstract

The production of vaccines of biological origin presents a tremendous challenge for re-searchers. In this context, animal cell cultures are an excellent alternative for the isolation and production of biologicals against several viruses, since they have an affinity with viruses and a great capacity for their replicability. Different variables have been studied to know the system’s ideal parameters, allowing it to obtain profitable and competitive products. Consequently, this work fo-cuses its efforts on evaluating an alternative for producing an anti‐influenza biological from MDCK cells using SuperPro Designer v8.0 software. The process uses the DMEN culture medium supple-mented with nutrients as raw material for cell development; the MDCK cells were obtained from a potential scale‐up with a final working volume of 500 L, four days of residence time, inoculum volume of 10%, and continuous working mode with up to a total of 7400 h/Yr of work. The scheme has the necessary equipment for the vaccine’s production, infection, and manufacture with yields of up to 416,698 units/h. In addition, it was estimated to be economically viable to produce recom-binant vaccines with competitive prices of up to 0.31 USD/unit.
2022
cell culture; developing countries; modeling process; public health; SuperPro Designer®
01 Pubblicazione su rivista::01a Articolo in rivista
A simulation analysis of an influenza vaccine production plant in areas of high humanitarian flow. A preliminary study for the region of norte de santander (colombia) / Contreras-ropero, J. E.; Ruiz-roa, S. L.; Garcia-martinez, J. B.; Urbina-suarez, N. A.; Lopez-barrera, G. L.; Barajas-solano, A. F.; Zuorro, A.. - In: APPLIED SCIENCES. - ISSN 2076-3417. - 12:1(2022). [10.3390/app12010183]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1667601
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