Microglial cells polarized towards a proinflammatory phenotype are considered the main cellular players of neuroinflammation, underlying several neurodegenerative diseases. Many studies have suggested that imbalance of the gut microbial composition is associated with an increase in the pro-inflammatory cytokines and oxidative stress that underlie chronic neuroinflammatory diseases, and perturbations to the gut microbiota were detected in neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease. The importance of gut-brain axis has been uncovered and the relevance of an appropriate microbiota balance has been highlighted. Probiotic treatment, rebalancing the gut microbioma, may reduce inflammation. We show that Milmed yeast, obtained from S. cerevisiae after exposure to electromagnetic millimeter wavelengths, induces a reversal of LPS-M1 polarized microglia towards an anti-inflammatory phenotype, as demonstrated morphologically by the recovery of resting phenotype by microglia, by the decrease in the mRNAs of IL-1β, IL-6, TNF-α and in the expression of iNOS. Moreover, Milmed stimulated the secretion of IL-10 and the expression of Arginase-1, cell markers of M2 anti-inflammatory polarized cells. The present findings data suggest that Milmed may be considered to be a probiotic with diversified anti-inflammatory activity, capable of directing the polarization of microglial cells.

Milmed yeast alters the LPS-induced M1 microglia cells to Form M2 anti-inflammatory phenotype / Armeli, Federica; Mengoni, Beatrice; Maggi, Elisa; Mazzoni, Cristina; Preziosi, Adele; Mancini, Patrizia; Businaro, Rita; Lenz, Thomas; Archer, TREVOR CORNELIUS STUART. - In: BIOMEDICINES. - ISSN 2227-9059. - 10:12(2022). [10.3390/biomedicines10123116]

Milmed yeast alters the LPS-induced M1 microglia cells to Form M2 anti-inflammatory phenotype

Federica Armeli;Beatrice Mengoni;Elisa Maggi;Cristina Mazzoni;Adele Preziosi;Patrizia Mancini;Rita Businaro
;
Trevor Archer
2022

Abstract

Microglial cells polarized towards a proinflammatory phenotype are considered the main cellular players of neuroinflammation, underlying several neurodegenerative diseases. Many studies have suggested that imbalance of the gut microbial composition is associated with an increase in the pro-inflammatory cytokines and oxidative stress that underlie chronic neuroinflammatory diseases, and perturbations to the gut microbiota were detected in neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease. The importance of gut-brain axis has been uncovered and the relevance of an appropriate microbiota balance has been highlighted. Probiotic treatment, rebalancing the gut microbioma, may reduce inflammation. We show that Milmed yeast, obtained from S. cerevisiae after exposure to electromagnetic millimeter wavelengths, induces a reversal of LPS-M1 polarized microglia towards an anti-inflammatory phenotype, as demonstrated morphologically by the recovery of resting phenotype by microglia, by the decrease in the mRNAs of IL-1β, IL-6, TNF-α and in the expression of iNOS. Moreover, Milmed stimulated the secretion of IL-10 and the expression of Arginase-1, cell markers of M2 anti-inflammatory polarized cells. The present findings data suggest that Milmed may be considered to be a probiotic with diversified anti-inflammatory activity, capable of directing the polarization of microglial cells.
2022
M1/M2 polarization; anti-inflammatory; microbiota; microglia; millimeter wavelength; neuroinflammation; yeast
01 Pubblicazione su rivista::01a Articolo in rivista
Milmed yeast alters the LPS-induced M1 microglia cells to Form M2 anti-inflammatory phenotype / Armeli, Federica; Mengoni, Beatrice; Maggi, Elisa; Mazzoni, Cristina; Preziosi, Adele; Mancini, Patrizia; Businaro, Rita; Lenz, Thomas; Archer, TREVOR CORNELIUS STUART. - In: BIOMEDICINES. - ISSN 2227-9059. - 10:12(2022). [10.3390/biomedicines10123116]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1664474
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