Objective: To study the cell effects of raloxifene on uterine and leiomyoma tissue in postmenopausal women. Design: Prospective, randomized, double-blind, placebo-controlled study.Setting: Department of Obstetrics and Gynecology, University "Magna Graccia" of Catanzaro, Italy.Patient(S): Forty postmenopausal women affected by uterine leiomyomas and selected for hysterectomy. Intervention(s): Treatment for three cycles of 28 days with raloxifene at a dose of 180 mg/day orally (raloxifene group) or placebo tablets (3 tablets/day orally) (placebo group).Main Outcome Measure(s): Uterine and leiomyoma dimensions were measured in each subject at entry and before surgery. On leiomyomas and homologous myometrium the proliferating cell nuclear antigen (PCNA)positive cells/total cells (PCNA/TQ and the Bcl-2-positive cells/Bax-positive cells (Bcl-2/Bax) ratios (%), as proliferation and apoptotic indexes, respectively, were measured.Result(S): After treatment, uterine and leiomyoma sizes were significantly changed in comparison with baseline and the placebo group. PCNA/TC and Bcl-2/Bax ratios were significantly higher in leiomyomas than in homologous myometrium. A significant difference was detected in PCNA/TC between the myometrium of the raloxifene and control groups, whereas no difference was observed in the Bcl-2/Bax ratio. A significant difference in PCNAITC and Bcl-2/Bax ratios was detected in leiomyoma tissue between the raloxifene group and controls.Conclusion(s): In postmenopausal women, raloxifene administration reduces uterine Iciomyomas by exerting a cell antiproliferative and proapoptotic action. (c) 2005 by American Society for Reproductive Medicine.
Antiproliferative and proapoptotic effects of raloxifene on uterine leiomyomas in postmenopausal women / Palomba, Stefano; Orio, Francesco; Russo, Tiziana; Falbo, Angela; Tolino, Achille; Lombardi, Gaetano; Cimini, Vincenzo; Zullo, Fulvio. - In: FERTILITY AND STERILITY. - ISSN 0015-0282. - 84:1(2005), pp. 154-161. [10.1016/j.fertnstert.2004.12.058]
Antiproliferative and proapoptotic effects of raloxifene on uterine leiomyomas in postmenopausal women
Palomba, Stefano;
2005
Abstract
Objective: To study the cell effects of raloxifene on uterine and leiomyoma tissue in postmenopausal women. Design: Prospective, randomized, double-blind, placebo-controlled study.Setting: Department of Obstetrics and Gynecology, University "Magna Graccia" of Catanzaro, Italy.Patient(S): Forty postmenopausal women affected by uterine leiomyomas and selected for hysterectomy. Intervention(s): Treatment for three cycles of 28 days with raloxifene at a dose of 180 mg/day orally (raloxifene group) or placebo tablets (3 tablets/day orally) (placebo group).Main Outcome Measure(s): Uterine and leiomyoma dimensions were measured in each subject at entry and before surgery. On leiomyomas and homologous myometrium the proliferating cell nuclear antigen (PCNA)positive cells/total cells (PCNA/TQ and the Bcl-2-positive cells/Bax-positive cells (Bcl-2/Bax) ratios (%), as proliferation and apoptotic indexes, respectively, were measured.Result(S): After treatment, uterine and leiomyoma sizes were significantly changed in comparison with baseline and the placebo group. PCNA/TC and Bcl-2/Bax ratios were significantly higher in leiomyomas than in homologous myometrium. A significant difference was detected in PCNA/TC between the myometrium of the raloxifene and control groups, whereas no difference was observed in the Bcl-2/Bax ratio. A significant difference in PCNAITC and Bcl-2/Bax ratios was detected in leiomyoma tissue between the raloxifene group and controls.Conclusion(s): In postmenopausal women, raloxifene administration reduces uterine Iciomyomas by exerting a cell antiproliferative and proapoptotic action. (c) 2005 by American Society for Reproductive Medicine.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
