Striated muscle is a highly organized structure composed of well-defined anatomical domains with integrated but distinct assignments. So far, the lack of a direct correlation between tissue architecture and gene expression has limited our understanding of how each unit responds to physio-pathologic contexts. Here, we show how the combined use of spatially resolved transcriptomics and immunofluorescence can bridge this gap by enabling the unbiased identification of such domains and the characterization of their response to external perturbations. Using a spatiotemporal analysis, we follow changes in the transcriptome of specific domains in muscle in a model of denervation. Furthermore, our approach enables us to identify the spatial distribution and nerve dependence of atrophic signaling pathway and polyamine metabolism to glycolytic fibers. Indeed, we demonstrate that perturbations of polyamine pathway can affect muscle function. Our dataset serves as a resource for future studies of the mechanisms underlying skeletal muscle homeostasis and innervation.

Spatially resolved transcriptomics reveals innervation-responsive functional clusters in skeletal muscle / D'Ercole, Chiara; D'Angelo, Paolo; Ruggieri, Veronica; Proietti, Daisy; Virtanen, Laura; Parisi, Cristina; Riera, Carles Sanchez; Renzini, Alessandra; Macone, Alberto; Marzullo, Marta; Ciapponi, Laura; Bonvissuto, Davide; Sette, Claudio; Giordani, Lorenzo; Madaro, Luca. - In: CELL REPORTS. - ISSN 2211-1247. - 41:12(2022), pp. 1-23. [10.1016/j.celrep.2022.111861]

Spatially resolved transcriptomics reveals innervation-responsive functional clusters in skeletal muscle

D'Ercole, Chiara
Primo
;
D'Angelo, Paolo;Ruggieri, Veronica;Proietti, Daisy;Riera, Carles Sanchez;Renzini, Alessandra;Macone, Alberto;Marzullo, Marta;Ciapponi, Laura;Bonvissuto, Davide;Giordani, Lorenzo
Penultimo
;
Madaro, Luca
Ultimo
2022

Abstract

Striated muscle is a highly organized structure composed of well-defined anatomical domains with integrated but distinct assignments. So far, the lack of a direct correlation between tissue architecture and gene expression has limited our understanding of how each unit responds to physio-pathologic contexts. Here, we show how the combined use of spatially resolved transcriptomics and immunofluorescence can bridge this gap by enabling the unbiased identification of such domains and the characterization of their response to external perturbations. Using a spatiotemporal analysis, we follow changes in the transcriptome of specific domains in muscle in a model of denervation. Furthermore, our approach enables us to identify the spatial distribution and nerve dependence of atrophic signaling pathway and polyamine metabolism to glycolytic fibers. Indeed, we demonstrate that perturbations of polyamine pathway can affect muscle function. Our dataset serves as a resource for future studies of the mechanisms underlying skeletal muscle homeostasis and innervation.
2022
Amd1; Amd2; CP: molecular biology; smox; denervation; muscular atrophy; polyamine; putrescin; skeletal muscle; spatial transcriptomics
01 Pubblicazione su rivista::01a Articolo in rivista
Spatially resolved transcriptomics reveals innervation-responsive functional clusters in skeletal muscle / D'Ercole, Chiara; D'Angelo, Paolo; Ruggieri, Veronica; Proietti, Daisy; Virtanen, Laura; Parisi, Cristina; Riera, Carles Sanchez; Renzini, Alessandra; Macone, Alberto; Marzullo, Marta; Ciapponi, Laura; Bonvissuto, Davide; Sette, Claudio; Giordani, Lorenzo; Madaro, Luca. - In: CELL REPORTS. - ISSN 2211-1247. - 41:12(2022), pp. 1-23. [10.1016/j.celrep.2022.111861]
File allegati a questo prodotto
File Dimensione Formato  
D'Ercole_Spazially_2022.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 9.16 MB
Formato Adobe PDF
9.16 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1662908
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 9
social impact