Astaxanthin (ASX) is a carotenoid with several applications in the food and pharmaceutical fields which is recognised as having a higher physiological and commercial value when it is biologically rather than chemically sourced. This work experimentally investigates the bio-fractionation of biomass of the Paracoccus carotinifaciens bacterium by extraction with the switchable hydrophilicity solvent N,N-dimethyl cyclohexylamine (DMCHA) to obtain ASX and related carotenoids in separate form. This is done by means of a sequential process carried out in a single apparatus. DMCHA gave an extraction yield in terms of equivalent ASX extracted per unit mass of processed dry microbial biomass (62.5 %) higher than acetone (48 %) and ethanol (14 %). After the DMCHA was washed from the extract with carbonated water, 88.2 % of the extracted ASX was recovered. The possibility of extracting carotenoids with recycled solvent with a minor (-7.7%) efficiency drop was also demonstrated, as was the lower energy footprint of DMCHA-based than competing volatile solvent-based extraction processes.

An innovative simplified one-pot process for Astaxanthin purification from Paracoccus carotinifaciens / Sapone, Vittoria; Iannone, Annalaura; Alivernini, Alessio; Cicci, Agnese; Jessop, Philip G.; Bravi, Marco. - In: SEPARATION AND PURIFICATION TECHNOLOGY. - ISSN 1383-5866. - 308:(2022), p. 122843. [10.1016/j.seppur.2022.122843]

An innovative simplified one-pot process for Astaxanthin purification from Paracoccus carotinifaciens

Sapone, Vittoria
Primo
Investigation
;
Iannone, Annalaura
Formal Analysis
;
Cicci, Agnese
Conceptualization
;
Bravi, Marco
Ultimo
Supervision
2022

Abstract

Astaxanthin (ASX) is a carotenoid with several applications in the food and pharmaceutical fields which is recognised as having a higher physiological and commercial value when it is biologically rather than chemically sourced. This work experimentally investigates the bio-fractionation of biomass of the Paracoccus carotinifaciens bacterium by extraction with the switchable hydrophilicity solvent N,N-dimethyl cyclohexylamine (DMCHA) to obtain ASX and related carotenoids in separate form. This is done by means of a sequential process carried out in a single apparatus. DMCHA gave an extraction yield in terms of equivalent ASX extracted per unit mass of processed dry microbial biomass (62.5 %) higher than acetone (48 %) and ethanol (14 %). After the DMCHA was washed from the extract with carbonated water, 88.2 % of the extracted ASX was recovered. The possibility of extracting carotenoids with recycled solvent with a minor (-7.7%) efficiency drop was also demonstrated, as was the lower energy footprint of DMCHA-based than competing volatile solvent-based extraction processes.
2022
paracoccus carotinifaciens; carotenoids; switchable hydrophilicity; solvents; extraction; recovery; astaxanthin
01 Pubblicazione su rivista::01a Articolo in rivista
An innovative simplified one-pot process for Astaxanthin purification from Paracoccus carotinifaciens / Sapone, Vittoria; Iannone, Annalaura; Alivernini, Alessio; Cicci, Agnese; Jessop, Philip G.; Bravi, Marco. - In: SEPARATION AND PURIFICATION TECHNOLOGY. - ISSN 1383-5866. - 308:(2022), p. 122843. [10.1016/j.seppur.2022.122843]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1662805
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