Insecticide resistance is a major threat challenging the control of harmful insect species. The study of resistant phenotypes is, therefore, pivotal to understand molecular mechanisms underpinning insecticide resistance and plan effective control and resistance management strategies. Here, we further analysed the diflubenzuron (DFB)-resistant phenotype due to the point-mutation I1043M in the chitin-synthase 1 gene (chs1) in the mosquito Culex pipiens. By comparing susceptible and resistant strains of Cx. pipiens through DFB bioassays, molecular analyses and scanning electron microscopy, we showed that the I1043M-resistant mosquitoes have: (i) a striking level of DFB resistance (i.e., resistance ratio: 9006); (ii) a constitutive 11-fold over-expression of the chs1 gene; iii) enhanced cuticle thickness and cuticular chitin content. Culex pipiens is one of the most important vector species in Europe and the rapid spread of DFB resistance can threaten its control. Our results, by adding new data about the DFB-resistant phenotype, provide important information for the control and management of insecticide resistance.

Cuticle modifications and over-expression of the chitin-synthase gene in diflubenzuron-resistant phenotype / Lucchesi, Valentina; Grimaldi, Lorenzo; Mastrantonio, Valentina; Porretta, Daniele; Di Bella, Letizia; Ruspandini, Tania; Di Salvo, Martino Luigi; Vontas, John; Bellini, Romeo; Negri, Agata; Epis, Sara; Caccia, Silvia; Bandi, Claudio; Urbanelli, Sandra. - In: INSECTS. - ISSN 2075-4450. - 13:12(2022). [10.3390/insects13121109]

Cuticle modifications and over-expression of the chitin-synthase gene in diflubenzuron-resistant phenotype

Lucchesi, Valentina;Grimaldi, Lorenzo;Mastrantonio, Valentina
;
Porretta, Daniele;Di Bella, Letizia;Ruspandini, Tania;Di Salvo, Martino Luigi;Bellini, Romeo;Negri, Agata;Urbanelli, Sandra
2022

Abstract

Insecticide resistance is a major threat challenging the control of harmful insect species. The study of resistant phenotypes is, therefore, pivotal to understand molecular mechanisms underpinning insecticide resistance and plan effective control and resistance management strategies. Here, we further analysed the diflubenzuron (DFB)-resistant phenotype due to the point-mutation I1043M in the chitin-synthase 1 gene (chs1) in the mosquito Culex pipiens. By comparing susceptible and resistant strains of Cx. pipiens through DFB bioassays, molecular analyses and scanning electron microscopy, we showed that the I1043M-resistant mosquitoes have: (i) a striking level of DFB resistance (i.e., resistance ratio: 9006); (ii) a constitutive 11-fold over-expression of the chs1 gene; iii) enhanced cuticle thickness and cuticular chitin content. Culex pipiens is one of the most important vector species in Europe and the rapid spread of DFB resistance can threaten its control. Our results, by adding new data about the DFB-resistant phenotype, provide important information for the control and management of insecticide resistance.
2022
chitin synthesis inhibitors; chitin synthase; insect vectors; insecticide resistance; mosquitoes; pest control; resistant phenotype
01 Pubblicazione su rivista::01a Articolo in rivista
Cuticle modifications and over-expression of the chitin-synthase gene in diflubenzuron-resistant phenotype / Lucchesi, Valentina; Grimaldi, Lorenzo; Mastrantonio, Valentina; Porretta, Daniele; Di Bella, Letizia; Ruspandini, Tania; Di Salvo, Martino Luigi; Vontas, John; Bellini, Romeo; Negri, Agata; Epis, Sara; Caccia, Silvia; Bandi, Claudio; Urbanelli, Sandra. - In: INSECTS. - ISSN 2075-4450. - 13:12(2022). [10.3390/insects13121109]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1661439
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