Epidermal development and maintenance are finely regulated events requiring a strict balance between proliferation and differentiation. Alterations in these processes give rise to human disorders such as cancer or syndromes with skin and annexes defects, known as ectodermal dysplasias (EDs). Here, we studied the functional effects of two novel receptor-interacting protein kinase 4 (RIPK4) missense mutations identified in siblings with an autosomal recessive ED with cutaneous syndactyly, palmoplantar hyperkeratosis and orofacial synechiae. Clinical overlap with distinct EDs caused by mutations in transcription factors (i.e. p63 and interferon regulatory factor 6, IRF6) or nectin adhesion molecules was noticed. Impaired activity of the RIPK4 kinase resulted both in altered epithelial differentiation and defective cell adhesion. We showed that mutant RIPK4 resulted in loss of PVRL4/nectin-4 expression in patient epidermis and primary keratinocytes, and demonstrated that PVRL4 is transcriptionally regulated by IRF6, a RIPK4 phosphorylation target. In addition, defective RIPK4 altered desmosome morphology through modulation of plakophilin-1 and desmoplakin. In conclusion, this work implicates RIPK4 kinase function in the p63-IRF6 regulatory loop that controls the proliferation/differentiation switch and cell adhesion, with implications in ectodermal development and cancer. © The Author(s) 2022.

RIPK4 regulates cell-cell adhesion in epidermal development and homeostasis / Fortugno, Paola; Monetta, Rosanna; Belli, Manuel; Botti, Elisabetta; Angelucci, Francesco; Grazia Palmerini, Maria; Nottola, Stefania Annarita; De Luca, Chiara; Ceccarini, Marina; Salvatore, Marco; Bianchi, Luca; Macioce, Pompeo; Teson, Massimo; Ricci, Francesco; Undiagnosed Diseases Network, Italian; Macchiarelli, Guido; Didona, Biagio; Costanzo, Antonio; Castiglia, Daniele; Brancati, Francesco. - In: HUMAN MOLECULAR GENETICS. - ISSN 0964-6906. - 31:15(2022), pp. 2535-2547. [10.1093/hmg/ddac046]

RIPK4 regulates cell-cell adhesion in epidermal development and homeostasis

Stefania Annarita Nottola;
2022

Abstract

Epidermal development and maintenance are finely regulated events requiring a strict balance between proliferation and differentiation. Alterations in these processes give rise to human disorders such as cancer or syndromes with skin and annexes defects, known as ectodermal dysplasias (EDs). Here, we studied the functional effects of two novel receptor-interacting protein kinase 4 (RIPK4) missense mutations identified in siblings with an autosomal recessive ED with cutaneous syndactyly, palmoplantar hyperkeratosis and orofacial synechiae. Clinical overlap with distinct EDs caused by mutations in transcription factors (i.e. p63 and interferon regulatory factor 6, IRF6) or nectin adhesion molecules was noticed. Impaired activity of the RIPK4 kinase resulted both in altered epithelial differentiation and defective cell adhesion. We showed that mutant RIPK4 resulted in loss of PVRL4/nectin-4 expression in patient epidermis and primary keratinocytes, and demonstrated that PVRL4 is transcriptionally regulated by IRF6, a RIPK4 phosphorylation target. In addition, defective RIPK4 altered desmosome morphology through modulation of plakophilin-1 and desmoplakin. In conclusion, this work implicates RIPK4 kinase function in the p63-IRF6 regulatory loop that controls the proliferation/differentiation switch and cell adhesion, with implications in ectodermal development and cancer. © The Author(s) 2022.
2022
cell adhesion; cell adhesion molecules; ectodermal dysplasia; homeostasis; humans; interferon regulatory factors; keratinocytes; nectins; protein serine-threonine kinases
01 Pubblicazione su rivista::01a Articolo in rivista
RIPK4 regulates cell-cell adhesion in epidermal development and homeostasis / Fortugno, Paola; Monetta, Rosanna; Belli, Manuel; Botti, Elisabetta; Angelucci, Francesco; Grazia Palmerini, Maria; Nottola, Stefania Annarita; De Luca, Chiara; Ceccarini, Marina; Salvatore, Marco; Bianchi, Luca; Macioce, Pompeo; Teson, Massimo; Ricci, Francesco; Undiagnosed Diseases Network, Italian; Macchiarelli, Guido; Didona, Biagio; Costanzo, Antonio; Castiglia, Daniele; Brancati, Francesco. - In: HUMAN MOLECULAR GENETICS. - ISSN 0964-6906. - 31:15(2022), pp. 2535-2547. [10.1093/hmg/ddac046]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1658148
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