Motivated by the request to monitor the airborne pathogens, like viruses, using ultrasensitive vibrational spectroscopy, we propose a comparative infrared spectroscopic study of viral glycoprotein spikes (S) of MERS-CoV, SARS-CoV and SARS-CoV-2, for the first time, at least to our knowledge. The aim is the investigation of a part of the pathogen, in this case the structural S proteins and their complex secondary structure that match the protein functionality, observing the amide I band (1590–1730 cm−1) in serological ambient and their potential differences. We experimentally observed that the three viruses have S proteins with different secondary conformations. SARS-CoV-2 has a secondary conformation characterized by the formation of intermolecular β-sheet structures, linked to the ability to anchoring virus-human receptors. Moreover, we also proved that the conformation of SARS-CoV-2 strongly change by passing from an alkaline to mild acid environment. Instead, the results pave the way to use vibrational spectroscopy as an alternative monitoring approach, overcoming the limitations of conventional bio-chemical ones.

Infrared spectroscopy characterization of MERS-CoV, SARS-CoV and SARS-CoV-2 spike proteins for sensoristic platform / D’Arco, Annalisa; DI FABRIZIO, Marta; Mancini, Tiziana; Mosetti, Rosanna; Della Ventura, Giancarlo; Marcelli, Augusto; Petrarca, Massimo; Lupi, Stefano. - (2022), pp. 275-276. (Intervento presentato al convegno 108° CONGRESSO NAZIONALE Società Italiana di Fisica tenutosi a Milano).

Infrared spectroscopy characterization of MERS-CoV, SARS-CoV and SARS-CoV-2 spike proteins for sensoristic platform.

D’Arco Annalisa
;
Di Fabrizio Marta;Mancini Tiziana;Mosetti Rosanna;Petrarca Massimo;Lupi Stefano
2022

Abstract

Motivated by the request to monitor the airborne pathogens, like viruses, using ultrasensitive vibrational spectroscopy, we propose a comparative infrared spectroscopic study of viral glycoprotein spikes (S) of MERS-CoV, SARS-CoV and SARS-CoV-2, for the first time, at least to our knowledge. The aim is the investigation of a part of the pathogen, in this case the structural S proteins and their complex secondary structure that match the protein functionality, observing the amide I band (1590–1730 cm−1) in serological ambient and their potential differences. We experimentally observed that the three viruses have S proteins with different secondary conformations. SARS-CoV-2 has a secondary conformation characterized by the formation of intermolecular β-sheet structures, linked to the ability to anchoring virus-human receptors. Moreover, we also proved that the conformation of SARS-CoV-2 strongly change by passing from an alkaline to mild acid environment. Instead, the results pave the way to use vibrational spectroscopy as an alternative monitoring approach, overcoming the limitations of conventional bio-chemical ones.
2022
108° CONGRESSO NAZIONALE Società Italiana di Fisica
04 Pubblicazione in atti di convegno::04d Abstract in atti di convegno
Infrared spectroscopy characterization of MERS-CoV, SARS-CoV and SARS-CoV-2 spike proteins for sensoristic platform / D’Arco, Annalisa; DI FABRIZIO, Marta; Mancini, Tiziana; Mosetti, Rosanna; Della Ventura, Giancarlo; Marcelli, Augusto; Petrarca, Massimo; Lupi, Stefano. - (2022), pp. 275-276. (Intervento presentato al convegno 108° CONGRESSO NAZIONALE Società Italiana di Fisica tenutosi a Milano).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1657777
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