We have developed a confocal laser microscope operating in the wavelength range between 5.8 and 6.3 μm with a parallel confocal visible-light illumination channel for the study of light-sensitive membrane proteins. The measured diffraction-limited lateral resolution is 19 μm. Differences in the amide-I and II bands of proteins of the order of 10−5 can be detected with 30 ms time resolution using cryogen-free HgCdTe detectors and lock-in amplification at the QCL repetition rate. The microscope is suitable to study rhodopsins with a slow photocycle of several seconds.

A Quantum Cascade Laser Microscope for Time-resolved Study of Membrane Proteins

Temperini M. E.;Polito R.;Intze A.;Gillibert R.;Baldassarre L.;Giliberti V.
2022

Abstract

We have developed a confocal laser microscope operating in the wavelength range between 5.8 and 6.3 μm with a parallel confocal visible-light illumination channel for the study of light-sensitive membrane proteins. The measured diffraction-limited lateral resolution is 19 μm. Differences in the amide-I and II bands of proteins of the order of 10−5 can be detected with 30 ms time resolution using cryogen-free HgCdTe detectors and lock-in amplification at the QCL repetition rate. The microscope is suitable to study rhodopsins with a slow photocycle of several seconds.
978-1-7281-9427-1
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1657464
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