We have developed a confocal laser microscope operating in the wavelength range between 5.8 and 6.3 μm with a parallel confocal visible-light illumination channel for the study of light-sensitive membrane proteins. The measured diffraction-limited lateral resolution is 19 μm. Differences in the amide-I and II bands of proteins of the order of 10−5 can be detected with 30 ms time resolution using cryogen-free HgCdTe detectors and lock-in amplification at the QCL repetition rate. The microscope is suitable to study rhodopsins with a slow photocycle of several seconds.
A Quantum Cascade Laser Microscope for Time-resolved Study of Membrane Proteins / Ortolani, M.; Temperini, M. E.; Polito, R.; Intze, A.; Ciano, C.; Gillibert, R.; Baldassarre, L.; Giliberti, V.. - 2022-August:(2022), pp. 1-2. (Intervento presentato al convegno 2022 47th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz) tenutosi a Delft, Netherlands) [10.1109/IRMMW-THz50927.2022.9895503].
A Quantum Cascade Laser Microscope for Time-resolved Study of Membrane Proteins
Temperini M. E.;Polito R.;Intze A.;Gillibert R.;Baldassarre L.;Giliberti V.
2022
Abstract
We have developed a confocal laser microscope operating in the wavelength range between 5.8 and 6.3 μm with a parallel confocal visible-light illumination channel for the study of light-sensitive membrane proteins. The measured diffraction-limited lateral resolution is 19 μm. Differences in the amide-I and II bands of proteins of the order of 10−5 can be detected with 30 ms time resolution using cryogen-free HgCdTe detectors and lock-in amplification at the QCL repetition rate. The microscope is suitable to study rhodopsins with a slow photocycle of several seconds.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
