We apply polarized Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR) to study conformational changes of the prototype photosensitive protein bacteriorhodopsin (BR). The experiments are performed on hundreds-nanometer-thick films of stacked membrane patches embedding BR molecules. Moreover, we report our results on functionalization of silicon surfaces that are preliminary to further experiments involving faced-down silicon substrates on ATR crystal in order to increase the sensitivity down to very thin protein films.
Conformational changes of an oriented film of photosensitive proteins observed by polarized ATR infrared spectroscopy / Temperini, Me; Polito, R; Intze, A; Baldassarre, L; Giliberti, V; Ortolani, M. - 2021-August:(2021), pp. 1-2. (Intervento presentato al convegno 2021 46th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz) tenutosi a Chengdu, China) [10.1109/IRMMW-THz50926.2021.9567067].
Conformational changes of an oriented film of photosensitive proteins observed by polarized ATR infrared spectroscopy
Temperini, ME;Polito, R;Intze, A;Baldassarre, L;Giliberti, V;
2021
Abstract
We apply polarized Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR) to study conformational changes of the prototype photosensitive protein bacteriorhodopsin (BR). The experiments are performed on hundreds-nanometer-thick films of stacked membrane patches embedding BR molecules. Moreover, we report our results on functionalization of silicon surfaces that are preliminary to further experiments involving faced-down silicon substrates on ATR crystal in order to increase the sensitivity down to very thin protein films.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
