Mass spectrometry analysis of dynamics and interactions of the LPS translocon LptDE

The presence of lipopolysaccharide (LPS) in the outer leaflet of the outer membrane (OM) is essential for Gram-negative bacteria OM barrier function and for maintaining its cell integrity. As such, comprehensive information about its biosynthesis and translocation represents a successful strategy for the development of antibacterial drugs. LPS is a complex glycolipid, and probing its interactions with LPS transport (Lpt) proteins has been extremely challenging. However, mass spectrometry (MS) techniques have recently catalyzed tremendous advancements in the characterization of LPS transport (Lpt) proteins and probed associated conformational dynamics upon substrate binding. Here, we describe the application of MS methods to study the dynamics of LPS translocon LptDE in the presence of natural substrates and inhibitors.