The presence of lipopolysaccharide (LPS) in the outer leaflet of the outer membrane (OM) is essential for Gram-negative bacteria OM barrier function and for maintaining its cell integrity. As such, comprehensive information about its biosynthesis and translocation represents a successful strategy for the development of antibacterial drugs. LPS is a complex glycolipid, and probing its interactions with LPS transport (Lpt) proteins has been extremely challenging. However, mass spectrometry (MS) techniques have recently catalyzed tremendous advancements in the characterization of LPS transport (Lpt) proteins and probed associated conformational dynamics upon substrate binding. Here, we describe the application of MS methods to study the dynamics of LPS translocon LptDE in the presence of natural substrates and inhibitors.

Mass Spectrometry Analysis of Dynamics and Interactions of the LPS Translocon LptDE / Fiorentino, Francesco; Bolla, Jani R. - (2022), pp. 109-128. - METHODS IN MOLECULAR BIOLOGY. [10.1007/978-1-0716-2581-1_8].

Mass Spectrometry Analysis of Dynamics and Interactions of the LPS Translocon LptDE

Fiorentino, Francesco
Primo
;
2022

Abstract

The presence of lipopolysaccharide (LPS) in the outer leaflet of the outer membrane (OM) is essential for Gram-negative bacteria OM barrier function and for maintaining its cell integrity. As such, comprehensive information about its biosynthesis and translocation represents a successful strategy for the development of antibacterial drugs. LPS is a complex glycolipid, and probing its interactions with LPS transport (Lpt) proteins has been extremely challenging. However, mass spectrometry (MS) techniques have recently catalyzed tremendous advancements in the characterization of LPS transport (Lpt) proteins and probed associated conformational dynamics upon substrate binding. Here, we describe the application of MS methods to study the dynamics of LPS translocon LptDE in the presence of natural substrates and inhibitors.
2022
Lipopolysaccharide Transport
978-1-0716-2580-4
978-1-0716-2581-1
Gram-negative bacteria; LPS; Lipopolysaccharide; Mass spectrometry; Outer membrane; Transport; Anti-Bacterial Agents; Bacterial Outer Membrane Proteins; Biological Transport; Glycolipids; Mass Spectrometry; Escherichia coli Proteins; Lipopolysaccharides
02 Pubblicazione su volume::02a Capitolo o Articolo
Mass Spectrometry Analysis of Dynamics and Interactions of the LPS Translocon LptDE / Fiorentino, Francesco; Bolla, Jani R. - (2022), pp. 109-128. - METHODS IN MOLECULAR BIOLOGY. [10.1007/978-1-0716-2581-1_8].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1657354
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