Cholangiocarcinoma (CCA) is a tumor with high tendency to infi ltrate nerves. Recent stud ies highlighted a key role of Schwann cells (SC) in cancer progression. This aspect is still unin vestigated in CCA We observed through a 3D model of perineural invasion the neurotropism of a CCA cell line (HuCC-T1), towards sciatic nerve explants, while no migration was observed using the cholangiocyte controls (H69). Migration and invasion of HuCC-T1 is fostered by the SC conditioned media. Neither the HuCC-T1 nor the H69 control produce factors capable of modulating neuritogenesis in PC12. Western blots performed on HuCC-T1 cells incubated for 48h with conditioned media from SC show a downregulation of E-Caderin indicative of epithelial-mesenchimal transition (EMT) and an upregulation of the proliferating nuclear antigen (PCNA) as compared to the controls. Our data indicate that SC may regulate EMT, migration, invasion and proliferation in Chol angiocarcinoma. we will further investigate this phenomenon by looking for potential mechanisms and molecular pathways involved

Cancer-nerve crosstalk in human cholangiocarcinoma / DE FRANCHIS, Valerio; Petrungaro, Simonetta; Mandolini, Enrico; Somma, Francesca; Pompili, Elena; Filippini, Antonio; Gaudio, Eugenio; Fabrizi, Cinzia; Giampietri, Claudia. - In: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY. - ISSN 2038-5129. - 126:Suppl 1(2022), pp. 47-47. ((Intervento presentato al convegno 75° Congresso della Società Italiana di Anatomia e Istologia tenutosi a Padova.

Cancer-nerve crosstalk in human cholangiocarcinoma

Valerio de Franchis;Simonetta Petrungaro;Elena Pompili;Antonio Filippini;Eugenio Gaudio;Cinzia Fabrizi;Claudia Giampietri
2022

Abstract

Cholangiocarcinoma (CCA) is a tumor with high tendency to infi ltrate nerves. Recent stud ies highlighted a key role of Schwann cells (SC) in cancer progression. This aspect is still unin vestigated in CCA We observed through a 3D model of perineural invasion the neurotropism of a CCA cell line (HuCC-T1), towards sciatic nerve explants, while no migration was observed using the cholangiocyte controls (H69). Migration and invasion of HuCC-T1 is fostered by the SC conditioned media. Neither the HuCC-T1 nor the H69 control produce factors capable of modulating neuritogenesis in PC12. Western blots performed on HuCC-T1 cells incubated for 48h with conditioned media from SC show a downregulation of E-Caderin indicative of epithelial-mesenchimal transition (EMT) and an upregulation of the proliferating nuclear antigen (PCNA) as compared to the controls. Our data indicate that SC may regulate EMT, migration, invasion and proliferation in Chol angiocarcinoma. we will further investigate this phenomenon by looking for potential mechanisms and molecular pathways involved
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1655580
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