The pathophysiology of COVID-19-associated coagulopathy is complex and not fully understood. SARS-CoV-2 spike protein (SP) may activate platelets and interact with fibrin(ogen). We aimed to investigate whether isolated SP can be present in clots retrieved in COVID-19 patients with acute ischemic stroke (by mechanical thrombectomy) and myocardial infarction. In this pilot study, we could detect SP, but not nucleocapsid protein, on platelets of COVID-19 patients' thrombi. In addition, in all three COVID-19 thrombi analyzed for molecular biology, no SARS-CoV-2 RNA could be detected by real-time polymerase chain reaction. These data could support the hypothesis that free SP, besides the whole virus, may be the trigger of platelet activation and clot formation in COVID-19.

Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients / De Michele, Manuela; D'Amati, Giulia; Leopizzi, Martina; Iacobucci, Marta; Berto, Irene; Lorenzano, Svetlana; Mazzuti, Laura; Turriziani, Ombretta; Schiavo, Oscar G; Toni, Danilo. - In: JOURNAL OF HEMATOLOGY & ONCOLOGY. - ISSN 1756-8722. - 15:1(2022), p. 108. [10.1186/s13045-022-01329-w]

Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients

De Michele, Manuela;d'Amati, Giulia;Leopizzi, Martina;Iacobucci, Marta;Berto, Irene;Lorenzano, Svetlana;Mazzuti, Laura;Turriziani, Ombretta;Schiavo, Oscar G;Toni, Danilo
2022

Abstract

The pathophysiology of COVID-19-associated coagulopathy is complex and not fully understood. SARS-CoV-2 spike protein (SP) may activate platelets and interact with fibrin(ogen). We aimed to investigate whether isolated SP can be present in clots retrieved in COVID-19 patients with acute ischemic stroke (by mechanical thrombectomy) and myocardial infarction. In this pilot study, we could detect SP, but not nucleocapsid protein, on platelets of COVID-19 patients' thrombi. In addition, in all three COVID-19 thrombi analyzed for molecular biology, no SARS-CoV-2 RNA could be detected by real-time polymerase chain reaction. These data could support the hypothesis that free SP, besides the whole virus, may be the trigger of platelet activation and clot formation in COVID-19.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1654207
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