Introduction: Oxysterols are cholesterol oxidation products and bioactive lipids involved in developmental signalling pathways, embryonic and postembryonic tissue patterning and homeostasis. The embryonic period is a very sensitive window of exposure to bisphenol A (BPA), hence the role of BPA on the levels of oxysterols in the very early stages of zebrafish embryogenesis is a relevant novel field of investigation. Objectives: To compare the role of BPA on oxysterols levels in zebrafish embryos at 8 and 24 h post fertilization (hpf) with cytochromes P450 (CYPs)-modulating chemicals (carbamazepine, ketoconazole, and hydrogen peroxide). Methods: Upon a dose range finding, zebrafish embryos were exposed to environmentally relevant (0.04 μM) and toxicological (17.5 μM) BPA concentrations. Seven oxysterols were profiled by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Results: Similarly to the CYPs-modulating chemicals, BPA caused: i) no significant changes at 8 hpf and ii) a dose-dependent increase of total oxysterols at 24 hpf, with 27-hydroxycholesterol as the most regulated oxysterol. Discussion: In the first day post-fertilization of the zebrafish embryos, the role of BPA alike a CYPs-modulating chemical was confirmed by the similar oxysterol changes observed with the already known CYPs-modulating chemicals.

Oxysterols profiles in zebrafish (Danio rerio) embryos exposed to bisphenol A / Vremere, A.; Merola, C.; Fanti, F.; Sergi, M.; Perugini, M.; Compagnone, D.; Mikhail, M.; Lorenzetti, S.; Amorena, M.. - In: FOOD AND CHEMICAL TOXICOLOGY. - ISSN 0278-6915. - 165:(2022), p. 113166. [10.1016/j.fct.2022.113166]

Oxysterols profiles in zebrafish (Danio rerio) embryos exposed to bisphenol A

Sergi M.;Compagnone D.;
2022

Abstract

Introduction: Oxysterols are cholesterol oxidation products and bioactive lipids involved in developmental signalling pathways, embryonic and postembryonic tissue patterning and homeostasis. The embryonic period is a very sensitive window of exposure to bisphenol A (BPA), hence the role of BPA on the levels of oxysterols in the very early stages of zebrafish embryogenesis is a relevant novel field of investigation. Objectives: To compare the role of BPA on oxysterols levels in zebrafish embryos at 8 and 24 h post fertilization (hpf) with cytochromes P450 (CYPs)-modulating chemicals (carbamazepine, ketoconazole, and hydrogen peroxide). Methods: Upon a dose range finding, zebrafish embryos were exposed to environmentally relevant (0.04 μM) and toxicological (17.5 μM) BPA concentrations. Seven oxysterols were profiled by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Results: Similarly to the CYPs-modulating chemicals, BPA caused: i) no significant changes at 8 hpf and ii) a dose-dependent increase of total oxysterols at 24 hpf, with 27-hydroxycholesterol as the most regulated oxysterol. Discussion: In the first day post-fertilization of the zebrafish embryos, the role of BPA alike a CYPs-modulating chemical was confirmed by the similar oxysterol changes observed with the already known CYPs-modulating chemicals.
2022
Bisphenol A; Carbamazepine; Hydrogen peroxide; Ketoconazole; Oxysterols; Zebrafish embryos; Animals; Benzhydryl Compounds; Embryo; Nonmammalian; Phenols; Tandem Mass Spectrometry; Zebrafish; Oxysterols; Water Pollutants; Chemical
01 Pubblicazione su rivista::01a Articolo in rivista
Oxysterols profiles in zebrafish (Danio rerio) embryos exposed to bisphenol A / Vremere, A.; Merola, C.; Fanti, F.; Sergi, M.; Perugini, M.; Compagnone, D.; Mikhail, M.; Lorenzetti, S.; Amorena, M.. - In: FOOD AND CHEMICAL TOXICOLOGY. - ISSN 0278-6915. - 165:(2022), p. 113166. [10.1016/j.fct.2022.113166]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1653238
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