tThe critical role of acute inflammatory processes is recognized in many chronic diseases; a key point inmolecular mechanisms of acute inflammation resolution is represented by a new group of pro-resolvinglipid mediators that include distinct families of molecules: lipoxins, resolvins, protectins and maresins,collectively termed “specialized pro-resolving mediators” (SPMs). In particular, resolvins are active in thepicogram to nanogram dose range, whereby they can directly modulate a plethora of anti-inflammatoryresponses. The presented method proposes an analytical protocol able to extract and to quantify 6 dif-ferent resolvins from 3 different matrices (plasma, cells and exudates). The method, validated accordingto the EMA guideline for bioanalysis, exhibited good precision (1%–20%) and accuracy (2%–20%). In par-ticular, the combination of two different sample preparation techniques, Liquid-Liquid Extraction (LLE)and micro-Solid Phase Extraction (SPE), applied for the first on this class of molecules, used for theextraction and clean-up respectively, led to high enrichment factor (20 fold) and consequently a highsensitivity (LOQ between 1 and 38 pg mL−1); moreover the validation data proved the versatility of SPEas clean-up tool as it was capable to manage huge enrichment factor without negatively affect accuracyand precision of analysis.

μSPE followed by HPLC–MS/MS for the determination of series D and E resolvins in biological matrices / Fanti, Federico; Oliva, Eleonora; Tortolani, Daniel; Di Meo, Camilla; Fava, Marina; Leuti, Alessandro; Rapino, Cinzia; Sergi, Manuel; Maccarrone, Mauro; Compagnone, Dario. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 203:(2021), pp. 1-9. [10.1016/j.jpba.2021.114181]

μSPE followed by HPLC–MS/MS for the determination of series D and E resolvins in biological matrices

Oliva, Eleonora;Sergi, Manuel;Compagnone, Dario
2021

Abstract

tThe critical role of acute inflammatory processes is recognized in many chronic diseases; a key point inmolecular mechanisms of acute inflammation resolution is represented by a new group of pro-resolvinglipid mediators that include distinct families of molecules: lipoxins, resolvins, protectins and maresins,collectively termed “specialized pro-resolving mediators” (SPMs). In particular, resolvins are active in thepicogram to nanogram dose range, whereby they can directly modulate a plethora of anti-inflammatoryresponses. The presented method proposes an analytical protocol able to extract and to quantify 6 dif-ferent resolvins from 3 different matrices (plasma, cells and exudates). The method, validated accordingto the EMA guideline for bioanalysis, exhibited good precision (1%–20%) and accuracy (2%–20%). In par-ticular, the combination of two different sample preparation techniques, Liquid-Liquid Extraction (LLE)and micro-Solid Phase Extraction (SPE), applied for the first on this class of molecules, used for theextraction and clean-up respectively, led to high enrichment factor (20 fold) and consequently a highsensitivity (LOQ between 1 and 38 pg mL−1); moreover the validation data proved the versatility of SPEas clean-up tool as it was capable to manage huge enrichment factor without negatively affect accuracyand precision of analysis.
2021
resolvins; biological matrices; liquid-liquid extraction; micro-SPE; HPLC–MS/MS
01 Pubblicazione su rivista::01a Articolo in rivista
μSPE followed by HPLC–MS/MS for the determination of series D and E resolvins in biological matrices / Fanti, Federico; Oliva, Eleonora; Tortolani, Daniel; Di Meo, Camilla; Fava, Marina; Leuti, Alessandro; Rapino, Cinzia; Sergi, Manuel; Maccarrone, Mauro; Compagnone, Dario. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 203:(2021), pp. 1-9. [10.1016/j.jpba.2021.114181]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1653235
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