Gene delivery is the basis for developing gene therapies that, in the future, may be able to cure virtually any disease, including viral infections. The use of short interfering RNAs (siRNAs) targeting viral replication is a novel strategy for treating HIV-1 infection. In this study, we prepared chitosan particles containing siRNA tat/rev via ionotropic gelation. Chitosan-based particles were efficiently internalized by cells, as evidenced by fluorescence microscopy. The antiviral effect of chitosan-based particles was studied on the C8166 cell line infected with HIV-1 and compared with the use of commercial liposomes (ESCORT). A significant reduction in HIV replication was also observed in cells treated with empty chitosan particles, suggesting that chitosan may interfere with the early steps of the HIV life cycle and have a synergic effect with siRNA to reduce viral replication significantly.

siRNA transfection mediated by chitosan microparticles for the treatment of HIV-1 infection of human cell lines / Chronopoulou, Laura; Falasca, Francesca; Di Fonzo, Federica; Turriziani, Ombretta; Palocci, Cleofe. - In: MATERIALS. - ISSN 1996-1944. - 15:15(2022). [10.3390/ma15155340]

siRNA transfection mediated by chitosan microparticles for the treatment of HIV-1 infection of human cell lines

Chronopoulou, Laura;Falasca, Francesca;Di Fonzo, Federica;Turriziani, Ombretta;Palocci, Cleofe
2022

Abstract

Gene delivery is the basis for developing gene therapies that, in the future, may be able to cure virtually any disease, including viral infections. The use of short interfering RNAs (siRNAs) targeting viral replication is a novel strategy for treating HIV-1 infection. In this study, we prepared chitosan particles containing siRNA tat/rev via ionotropic gelation. Chitosan-based particles were efficiently internalized by cells, as evidenced by fluorescence microscopy. The antiviral effect of chitosan-based particles was studied on the C8166 cell line infected with HIV-1 and compared with the use of commercial liposomes (ESCORT). A significant reduction in HIV replication was also observed in cells treated with empty chitosan particles, suggesting that chitosan may interfere with the early steps of the HIV life cycle and have a synergic effect with siRNA to reduce viral replication significantly.
2022
HIV-1; chitosan; gene delivery; polyplexes; siRNA; viral inhibition
01 Pubblicazione su rivista::01a Articolo in rivista
siRNA transfection mediated by chitosan microparticles for the treatment of HIV-1 infection of human cell lines / Chronopoulou, Laura; Falasca, Francesca; Di Fonzo, Federica; Turriziani, Ombretta; Palocci, Cleofe. - In: MATERIALS. - ISSN 1996-1944. - 15:15(2022). [10.3390/ma15155340]
File allegati a questo prodotto
File Dimensione Formato  
Chronopoulou_siRNA-transfection_2022.pdf

accesso aperto

Note: manuscript
Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 4.75 MB
Formato Adobe PDF
4.75 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1652980
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 5
social impact