Objective: to assess the influence of SARS-CoV-2 mRNA vaccine on B-cell phenotypes in systemic sclerosis (SSc). Methods: peripheral blood B-cell subpopulations were evaluated before (t1) and 3 months (t3) after the second dose of vaccine in 28 SSc patients. Peripheral blood B-cell subpopulations were evaluated in 21 healthy controls (HCs) only at t1. Anti-spike IgG levels were evaluated at t3 in both cohorts. Results: SSc patients presented higher naive, double-negative, and CD21low B cells compared to HCs. IgM-memory and switched-memory B cells were lower in SSc patients than HCs. No differences in anti-spike IgG levels after vaccination were observed between SSc patients and HCs. Anti-spike IgG levels after vaccination were lower in SSc patients with increased CD21low B cells at baseline compared to SSc patients with normal CD21low B cells. A positive correlation was found between IgG levels and naive B cells. A negative linear correlation was shown between IgG levels and IgM-memory, switched-memory, double-negative, and CD21low B cells. Conclusions: SARS-CoV-2 mRNA vaccine response is normal in SSc patients not undergoing immunosuppressive therapy. The normal number of naive B cells is a positive marker of antibody response. The increased percentage of CD21low B cells represents a negative marker of antibody response.

The effect of SARS-CoV-2 vaccination on B-cell phenotype in systemic sclerosis patients / Pellicano, Chiara; Colalillo, Amalia; Basile, Valerio; Marino, Mariapaola; Basile, Umberto; LA GUALANA, Francesca; Mezzaroma, Ivano; Visentini, Marcella; Rosato, Edoardo. - In: JOURNAL OF PERSONALIZED MEDICINE. - ISSN 2075-4426. - 12:9(2022), pp. 1-11. [10.3390/jpm12091420]

The effect of SARS-CoV-2 vaccination on B-cell phenotype in systemic sclerosis patients

Chiara Pellicano;Amalia Colalillo;Francesca La Gualana;Ivano Mezzaroma;Marcella Visentini;Edoardo Rosato
2022

Abstract

Objective: to assess the influence of SARS-CoV-2 mRNA vaccine on B-cell phenotypes in systemic sclerosis (SSc). Methods: peripheral blood B-cell subpopulations were evaluated before (t1) and 3 months (t3) after the second dose of vaccine in 28 SSc patients. Peripheral blood B-cell subpopulations were evaluated in 21 healthy controls (HCs) only at t1. Anti-spike IgG levels were evaluated at t3 in both cohorts. Results: SSc patients presented higher naive, double-negative, and CD21low B cells compared to HCs. IgM-memory and switched-memory B cells were lower in SSc patients than HCs. No differences in anti-spike IgG levels after vaccination were observed between SSc patients and HCs. Anti-spike IgG levels after vaccination were lower in SSc patients with increased CD21low B cells at baseline compared to SSc patients with normal CD21low B cells. A positive correlation was found between IgG levels and naive B cells. A negative linear correlation was shown between IgG levels and IgM-memory, switched-memory, double-negative, and CD21low B cells. Conclusions: SARS-CoV-2 mRNA vaccine response is normal in SSc patients not undergoing immunosuppressive therapy. The normal number of naive B cells is a positive marker of antibody response. The increased percentage of CD21low B cells represents a negative marker of antibody response.
2022
B cells; COVID-19; humoral response; mRNA vaccine; systemic sclerosis
01 Pubblicazione su rivista::01a Articolo in rivista
The effect of SARS-CoV-2 vaccination on B-cell phenotype in systemic sclerosis patients / Pellicano, Chiara; Colalillo, Amalia; Basile, Valerio; Marino, Mariapaola; Basile, Umberto; LA GUALANA, Francesca; Mezzaroma, Ivano; Visentini, Marcella; Rosato, Edoardo. - In: JOURNAL OF PERSONALIZED MEDICINE. - ISSN 2075-4426. - 12:9(2022), pp. 1-11. [10.3390/jpm12091420]
File allegati a questo prodotto
File Dimensione Formato  
Pellicano_Effect_2022.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 1.06 MB
Formato Adobe PDF
1.06 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1652817
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact