The growing spread of pathogens, caused by anthropogenic activities, pushes the interest of the scientific community towards developing biosensors with improved performance for rapid, simple, and on-site pathogen detection. In this study, we present and discuss a label-free gold nanorod (Au NR) array for the rapid detection of Escherichia coli cells in water, resulting in an effective optical transducer, based on the phenomenon of localized surface plasmon resonance (LSPR). Au NRs with different aspect ratios are func- tionalized with a suitable antibody by an electrostatic-linking method, resulting in two different Au NR- based bioconjugates. We investigate the ability of the two bioconjugates to detect and spectroscopically recognize E. coli cells dispersed in water by specific antigen–antibody interaction. The results allow selecting the Au NR morphology more suited for preparing the Au NR bioactive array on a glass substrate with excellent optical and morphological properties. The antibody-functionalized Au NR array can detect E. coli cells with high sensitivity and a limit of detection of 8.4 CFU mL−1, resulting in an excellent label-free spectroscopic biosensor. In addition, the multicolor thermoplasmonic properties of the Au NR array, trig- gered by appropriate light sources, are suited to enable on-demand photothermal disinfection, thus pro- viding an extraordinary capacity for the biosensor to be both disinfected and, more importantly, reutilized.

Label-free and reusable antibody-functionalized gold nanorod arrays for the rapid detection of Escherichia coli cells in a water dispersion

Daniela De Biase;Federica Zaccagnini;Vanessa Verrina;Selenia Miglietta;Vincenzo Petrozza;Luciano De Sio
2022

Abstract

The growing spread of pathogens, caused by anthropogenic activities, pushes the interest of the scientific community towards developing biosensors with improved performance for rapid, simple, and on-site pathogen detection. In this study, we present and discuss a label-free gold nanorod (Au NR) array for the rapid detection of Escherichia coli cells in water, resulting in an effective optical transducer, based on the phenomenon of localized surface plasmon resonance (LSPR). Au NRs with different aspect ratios are func- tionalized with a suitable antibody by an electrostatic-linking method, resulting in two different Au NR- based bioconjugates. We investigate the ability of the two bioconjugates to detect and spectroscopically recognize E. coli cells dispersed in water by specific antigen–antibody interaction. The results allow selecting the Au NR morphology more suited for preparing the Au NR bioactive array on a glass substrate with excellent optical and morphological properties. The antibody-functionalized Au NR array can detect E. coli cells with high sensitivity and a limit of detection of 8.4 CFU mL−1, resulting in an excellent label-free spectroscopic biosensor. In addition, the multicolor thermoplasmonic properties of the Au NR array, trig- gered by appropriate light sources, are suited to enable on-demand photothermal disinfection, thus pro- viding an extraordinary capacity for the biosensor to be both disinfected and, more importantly, reutilized.
File allegati a questo prodotto
File Dimensione Formato  
Petronella_Label-free_2022.pdf

accesso aperto

Note: Label-free and reusable antibody-functionalized gold nanorod arrays for the rapid detection of Escherichia coli cells in a water dispersion
Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 4.14 MB
Formato Adobe PDF
4.14 MB Adobe PDF Visualizza/Apri PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1652009
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 2
social impact