In this paper we present an improved version of a method for the automatic analysis of flow cytometric DNA histograms from samples containing a mixture of two cell populations. The procedure is tested against two sets of ad hoc experimental data, obtained by mixing cultures of cell lines in different known proportions. The potentialities of the method are enlightened and discussed with regard to its capability of recovering the population percentages, the DNA index and the G0/G1, S, G2+M phase fractions of each population. On the basis of the obtained results, the procedure appears to be a promising tool in the flow cytometric data analysis and, in particular, in problems of diagnosis and prognosis of tumor diseases.
Automatic Analysis of Flow Cytometrically Determined DNA Distributions in the Presence of Abnormal Stemlines / Bruni, Carlo; Capurso, L; Koch, G; Koch, C; Lampariello, F; Lucidi, Stefano; Teodori, L.. - In: MATHEMATICAL MODELLING. - ISSN 0270-0255. - STAMPA. - 7:(1986), pp. 1325-1338. [10.1016/0270-0255(86)90083-7]
Automatic Analysis of Flow Cytometrically Determined DNA Distributions in the Presence of Abnormal Stemlines
BRUNI, Carlo;LUCIDI, Stefano;
1986
Abstract
In this paper we present an improved version of a method for the automatic analysis of flow cytometric DNA histograms from samples containing a mixture of two cell populations. The procedure is tested against two sets of ad hoc experimental data, obtained by mixing cultures of cell lines in different known proportions. The potentialities of the method are enlightened and discussed with regard to its capability of recovering the population percentages, the DNA index and the G0/G1, S, G2+M phase fractions of each population. On the basis of the obtained results, the procedure appears to be a promising tool in the flow cytometric data analysis and, in particular, in problems of diagnosis and prognosis of tumor diseases.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
