The measurement of biological tissues’ dielectric properties plays a crucial role in determining the state of health, and recent studies have reported microwave biosensing to be an innovative method with great potential in this field. Research has been conducted from the tissue level to the cellular level but, to date, cellular adhesion has never been considered. In addition, conventional systems for diagnosing tumor aggressiveness, such as a biopsy, are rather expensive and invasive. Here, we propose a novel microwave approach for biosensing adherent cancer cells with different malignancy degrees. A circular patch resonator was designed adjusting its structure to a standard Petri dish and a network analyzer was employed. Then, the resonator was realized and used to test two groups of different cancer cell lines, based on various tumor types and aggressiveness: low- and high-aggressive osteosarcoma cell lines (SaOS-2 and 143B, respectively), and low- and high-aggressive breast cancer cell lines (MCF-7 and MDA-MB-231, respectively). The experimental results showed that the sensitivity of the sensor was high, in particular when measuring the resonant frequency. Finally, the sensor showed a good ability to distinguish low-metastatic and high-metastatic cells, paving the way to the development of more complex measurement systems for noninvasive tissue diagnosis.

A novel microwave resonant sensor for measuring cancer cell line aggressiveness / D’Alvia, Livio; Carraro, Serena; Peruzzi, Barbara; Urciuoli, Enrica; Palla, Luigi; Del Prete, Zaccaria; Rizzuto, Emanuele. - In: SENSORS. - ISSN 1424-8220. - 22:12(2022), pp. 1-12. [10.3390/s22124383]

A novel microwave resonant sensor for measuring cancer cell line aggressiveness

D’Alvia, Livio
Primo
;
Carraro, Serena;Palla, Luigi;Del Prete, Zaccaria;Rizzuto, Emanuele
2022

Abstract

The measurement of biological tissues’ dielectric properties plays a crucial role in determining the state of health, and recent studies have reported microwave biosensing to be an innovative method with great potential in this field. Research has been conducted from the tissue level to the cellular level but, to date, cellular adhesion has never been considered. In addition, conventional systems for diagnosing tumor aggressiveness, such as a biopsy, are rather expensive and invasive. Here, we propose a novel microwave approach for biosensing adherent cancer cells with different malignancy degrees. A circular patch resonator was designed adjusting its structure to a standard Petri dish and a network analyzer was employed. Then, the resonator was realized and used to test two groups of different cancer cell lines, based on various tumor types and aggressiveness: low- and high-aggressive osteosarcoma cell lines (SaOS-2 and 143B, respectively), and low- and high-aggressive breast cancer cell lines (MCF-7 and MDA-MB-231, respectively). The experimental results showed that the sensitivity of the sensor was high, in particular when measuring the resonant frequency. Finally, the sensor showed a good ability to distinguish low-metastatic and high-metastatic cells, paving the way to the development of more complex measurement systems for noninvasive tissue diagnosis.
2022
measurement dielectric properties; biosensor; microwaves; noninvasive measurements; cancer cell lines; cancer aggressiveness; osteosarcoma; breast cancer
01 Pubblicazione su rivista::01a Articolo in rivista
A novel microwave resonant sensor for measuring cancer cell line aggressiveness / D’Alvia, Livio; Carraro, Serena; Peruzzi, Barbara; Urciuoli, Enrica; Palla, Luigi; Del Prete, Zaccaria; Rizzuto, Emanuele. - In: SENSORS. - ISSN 1424-8220. - 22:12(2022), pp. 1-12. [10.3390/s22124383]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1644248
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