: Modification of surface silanols is a topic of interest in the preparation of organo-functionalized silica particles. Herein, two novel contributions, mainly focused on separation science, were presented: i) the horizontal polymerization on silica surface by using the vinyl-triacetoxy silane and ii) a preparation of cysteine-based stationary phase via photo-click thiol-ene coupling. In the first derivatization step, the vinyl-triacetoxy silane was employed instead of conventional trichloro vinyl one. The one-step synthetic procedure needed imidazole as an activating agent in addition to the silica hydration. Modified silica particles offer a high loading of vinyl fragments and an extensive passivation of silanols such as to not require a subsequent end-capping procedure. The structural morphology of media was deeper characterized by combining infrared spectroscopy, solid-state nuclear magnetic resonance, and elemental analysis. A first application, the photo-click cysteine-based material was prepared by photo-click reaction and the stationary phase was employed in the separation of some conventional targets by hydrophilic interaction chromatography.

Preparation of a high-density vinyl silica gel to anchor cysteine via photo-click reaction and its applications in hydrophilic interaction chromatography / Ciogli, Alessia; Buonsenso, Fabio; Proietti, Noemi; Mazzoccanti, Giulia; Manetto, Simone; Calcaterra, Andrea; DE ANGELIS, Martina; Gasparrini, Francesco. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 1675:(2022). [10.1016/j.chroma.2022.463173]

Preparation of a high-density vinyl silica gel to anchor cysteine via photo-click reaction and its applications in hydrophilic interaction chromatography

Alessia Ciogli
;
Fabio Buonsenso;Giulia Mazzoccanti;Simone Manetto;Andrea Calcaterra;Martina De Angelis;Francesco Gasparrini
2022

Abstract

: Modification of surface silanols is a topic of interest in the preparation of organo-functionalized silica particles. Herein, two novel contributions, mainly focused on separation science, were presented: i) the horizontal polymerization on silica surface by using the vinyl-triacetoxy silane and ii) a preparation of cysteine-based stationary phase via photo-click thiol-ene coupling. In the first derivatization step, the vinyl-triacetoxy silane was employed instead of conventional trichloro vinyl one. The one-step synthetic procedure needed imidazole as an activating agent in addition to the silica hydration. Modified silica particles offer a high loading of vinyl fragments and an extensive passivation of silanols such as to not require a subsequent end-capping procedure. The structural morphology of media was deeper characterized by combining infrared spectroscopy, solid-state nuclear magnetic resonance, and elemental analysis. A first application, the photo-click cysteine-based material was prepared by photo-click reaction and the stationary phase was employed in the separation of some conventional targets by hydrophilic interaction chromatography.
2022
Horizontal polymerization; Photo-click reaction; Silica characterization; Solid-state NMR; Vinyl silica gel
01 Pubblicazione su rivista::01a Articolo in rivista
Preparation of a high-density vinyl silica gel to anchor cysteine via photo-click reaction and its applications in hydrophilic interaction chromatography / Ciogli, Alessia; Buonsenso, Fabio; Proietti, Noemi; Mazzoccanti, Giulia; Manetto, Simone; Calcaterra, Andrea; DE ANGELIS, Martina; Gasparrini, Francesco. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 1675:(2022). [10.1016/j.chroma.2022.463173]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1643176
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