CHARACTERIZATION OF PARASITE ANTIGENS FROM HUMAN HYDATID CYST FLUID BY SDS-PAGE AND IEF

Raffaele, D'Amelio; Pontesilli, O.; Dayal, R.; De Rosa, F.; Barnet, M.; Teggi, A.; Brighouse, G.; Lambert, P.H.

doi:10.1007/bf02123670

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Combining high resolution power of SDS-PAGE and IEF with the specific immunological recognition of a human antiserum directed against Echinococcus granulosus antigens, we could identify, in 4 hydatid cystic fluids of human origin, 4 antigens with a molecular weight in the range 32-13 KD, and an antigen of 200 KD which, in reducing conditions, gave 2 bands of 67 and 52 KD. In addition, mainly in one of the cystic fluids, there were at least another 4 specific non-reducible bands with a molecular weight ranging from 80 to 40 KD. Specific parasite antigens, which constitute not more than 3% of total protein content of the cystic fluid, migrate, in isoelectric focusing, from a pH of less than 5 to more than 8. © 1985 Springer-Verlag.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/16424

Titolo:	CHARACTERIZATION OF PARASITE ANTIGENS FROM HUMAN HYDATID CYST FLUID BY SDS-PAGE AND IEF
Autori interni:	D'AMELIO, Raffaele
Data di pubblicazione:	1985
Rivista:	MEDICAL MICROBIOLOGY AND IMMUNOLOGY
Abstract:	Combining high resolution power of SDS-PAGE and IEF with the specific immunological recognition of a human antiserum directed against Echinococcus granulosus antigens, we could identify, in 4 hydatid cystic fluids of human origin, 4 antigens with a molecular weight in the range 32-13 KD, and an antigen of 200 KD which, in reducing conditions, gave 2 bands of 67 and 52 KD. In addition, mainly in one of the cystic fluids, there were at least another 4 specific non-reducible bands with a molecular weight ranging from 80 to 40 KD. Specific parasite antigens, which constitute not more than 3% of total protein content of the cystic fluid, migrate, in isoelectric focusing, from a pH of less than 5 to more than 8. © 1985 Springer-Verlag.
Handle:	http://hdl.handle.net/11573/16424
Appare nelle tipologie:	01.a Pubblicazione su Rivista

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