Combining high resolution power of SDS-PAGE and IEF with the specific immunological recognition of a human antiserum directed against Echinococcus granulosus antigens, we could identify, in 4 hydatid cystic fluids of human origin, 4 antigens with a molecular weight in the range 32-13 KD, and an antigen of 200 KD which, in reducing conditions, gave 2 bands of 67 and 52 KD. In addition, mainly in one of the cystic fluids, there were at least another 4 specific non-reducible bands with a molecular weight ranging from 80 to 40 KD. Specific parasite antigens, which constitute not more than 3% of total protein content of the cystic fluid, migrate, in isoelectric focusing, from a pH of less than 5 to more than 8. © 1985 Springer-Verlag.
CHARACTERIZATION OF PARASITE ANTIGENS FROM HUMAN HYDATID CYST FLUID BY SDS-PAGE AND IEF / D'Amelio, Raffaele; O., Pontesilli; R., Dayal; F., De Rosa; M., Barnet; A., Teggi; G., Brighouse; P. H., Lambert. - In: MEDICAL MICROBIOLOGY AND IMMUNOLOGY. - ISSN 0300-8584. - 174:1(1985), pp. 43-50. [10.1007/bf02123670]
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Titolo: | CHARACTERIZATION OF PARASITE ANTIGENS FROM HUMAN HYDATID CYST FLUID BY SDS-PAGE AND IEF | |
Autori: | ||
Data di pubblicazione: | 1985 | |
Rivista: | ||
Citazione: | CHARACTERIZATION OF PARASITE ANTIGENS FROM HUMAN HYDATID CYST FLUID BY SDS-PAGE AND IEF / D'Amelio, Raffaele; O., Pontesilli; R., Dayal; F., De Rosa; M., Barnet; A., Teggi; G., Brighouse; P. H., Lambert. - In: MEDICAL MICROBIOLOGY AND IMMUNOLOGY. - ISSN 0300-8584. - 174:1(1985), pp. 43-50. [10.1007/bf02123670] | |
Handle: | http://hdl.handle.net/11573/16424 | |
Appartiene alla tipologia: | 01a Articolo in rivista |