Introduciton Amino-bisphosphonates are potent activators of human gamma delta T cells. The aim of our study was to evaluate the immunomodulating properties of a single-dose of zoledronic acid (ZA) on gamma delta T cells in a select group of disease-free breast cancer patients with osteopenia. Materials and methods Blood samples were obtained, from 23 patients, before and 7, 28, 56, 90 and 180 days after a single-dose (4 mg) of ZA and analyzed by flow cyometry. Results A significant decrease of the different gamma delta T cell subsets was observed: Naive (CD3+/Vdelta2+/CD45RA+/CD27+) after 180 days (P < 0.01); Central Memory (CD3+/Vdelta2+/CD45RA-CD27+) after 28 (P < 0.05), 90 (P < 0.01) and 180 days (P < 0.01); and Effector Memory (CD3+/Vdelta2+/CD45RA-/CD27-) after 56 (P < 0.01) and 90 (P < 0.05) days. Based on the observed gamma delta T cells kinetics patients could be divided in two groups: "responders" that showed a significant decrease in total numbers of gamma delta T cells and "non-responders" that showed no significant change. However, in vitro phosphoantigen stimulation of patients cells did not show significant differences in terms of IFN-gamma response by V delta 2 T cells. Conclusion We describe for the first time a long-lasting activation of effector subsets of gamma delta T cells in disease-free breast cancer patients after a single-dose of ZA. Our results highlight the need to further investigate the clinical significance of the immunomodulating properties of N-BPs.
In vivo effects of zoledronic acid on peripheral gamma delta T lymphocytes in early breast cancer patients / Santini, D; Martini, F; Fratto, Me; Galluzzo, S; Vincenzi, B; Agrati, C; Turchi, F; Piacentini, P; Rocci, L; Manavalan, Js; Tonini, G; Poccia, F. - In: CANCER IMMUNOLOGY, IMMUNOTHERAPY. - ISSN 0340-7004. - 58:1(2009), pp. 31-38. [10.1007/s00262-008-0521-6]
In vivo effects of zoledronic acid on peripheral gamma delta T lymphocytes in early breast cancer patients
Santini D;
2009
Abstract
Introduciton Amino-bisphosphonates are potent activators of human gamma delta T cells. The aim of our study was to evaluate the immunomodulating properties of a single-dose of zoledronic acid (ZA) on gamma delta T cells in a select group of disease-free breast cancer patients with osteopenia. Materials and methods Blood samples were obtained, from 23 patients, before and 7, 28, 56, 90 and 180 days after a single-dose (4 mg) of ZA and analyzed by flow cyometry. Results A significant decrease of the different gamma delta T cell subsets was observed: Naive (CD3+/Vdelta2+/CD45RA+/CD27+) after 180 days (P < 0.01); Central Memory (CD3+/Vdelta2+/CD45RA-CD27+) after 28 (P < 0.05), 90 (P < 0.01) and 180 days (P < 0.01); and Effector Memory (CD3+/Vdelta2+/CD45RA-/CD27-) after 56 (P < 0.01) and 90 (P < 0.05) days. Based on the observed gamma delta T cells kinetics patients could be divided in two groups: "responders" that showed a significant decrease in total numbers of gamma delta T cells and "non-responders" that showed no significant change. However, in vitro phosphoantigen stimulation of patients cells did not show significant differences in terms of IFN-gamma response by V delta 2 T cells. Conclusion We describe for the first time a long-lasting activation of effector subsets of gamma delta T cells in disease-free breast cancer patients after a single-dose of ZA. Our results highlight the need to further investigate the clinical significance of the immunomodulating properties of N-BPs.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
