In this paper, the implementation and main realization issues of a stimulated Raman scattering (SRS) microscope, obtained by the integration of a femtosecond SRS spectroscopy set-up with an optical microscope equipped with a scanning unit, are described. In order to characterize capabilities and limitations of SRS microscope, the optical resolution is measured and validation tests on polystyrene beads imaging are reported. In addition, taking account of strength of SRS vibrational contrast, label-free imaging of small biological structures, as such as lipid droplets (LDs), in adipocyte cells is carried out. The visualization of small LDs, with dimensions comparable to the optical lateral resolution of our system, is demonstrated.

Label-free imaging of small lipid droplets by femtosecond-stimulated Raman scattering microscopy / D'Arco, A.; Ferrara, M. A.; Indolfi, M.; Tufano, V.; Sirleto, L.. - In: JOURNAL OF NONLINEAR OPTICAL PHYSICS AND MATERIALS. - ISSN 0218-8635. - 26:4(2017). [10.1142/S0218863517500527]

Label-free imaging of small lipid droplets by femtosecond-stimulated Raman scattering microscopy

D'Arco A.;
2017

Abstract

In this paper, the implementation and main realization issues of a stimulated Raman scattering (SRS) microscope, obtained by the integration of a femtosecond SRS spectroscopy set-up with an optical microscope equipped with a scanning unit, are described. In order to characterize capabilities and limitations of SRS microscope, the optical resolution is measured and validation tests on polystyrene beads imaging are reported. In addition, taking account of strength of SRS vibrational contrast, label-free imaging of small biological structures, as such as lipid droplets (LDs), in adipocyte cells is carried out. The visualization of small LDs, with dimensions comparable to the optical lateral resolution of our system, is demonstrated.
2017
Coherent Raman Microscopy; label free imaging; nonlinear optics
01 Pubblicazione su rivista::01a Articolo in rivista
Label-free imaging of small lipid droplets by femtosecond-stimulated Raman scattering microscopy / D'Arco, A.; Ferrara, M. A.; Indolfi, M.; Tufano, V.; Sirleto, L.. - In: JOURNAL OF NONLINEAR OPTICAL PHYSICS AND MATERIALS. - ISSN 0218-8635. - 26:4(2017). [10.1142/S0218863517500527]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1640687
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