Hypothesis: Sodium deoxycholate is a natural bile salt produced by animals and fulfilling important physiological processes. It is also used as dispersive surfactant and building block for self-assembled architectures in biology and material science. Although long debated, the study of its self-assembly in water is hereto incomplete and the models of the known aggregates are still controversial. This background suggests a complex scenario likely missing of additional mesophases. Experiments: Electron and optical microscopy techniques were crossed with SAXS data for the research. Findings: Novel rod, sponge, vesicle, lamellae, nanotube phases and reversible transitions among them arise at conditions (concentration, pH, temperature, ionic strength, ionic composition) fitting the physiological working environment of sodium deoxycholate. These findings enlarge the perspective towards different directions. The integration of the previous literature with this work removes any interpretative contradiction since all the structures cover the entire spectrum of phases expected for surfactants, thus being explained according to the Israelachvili's scheme. It is not trivial that a single molecule can show such a high structural variability. This fact highlights a very versatile system. Probably it is not a coincidence that it occurs in a multitasking biomolecule. These results furnish fundamental knowledge to clarify the bile salts’ role in vivo.

Revealing the complex self-assembly behaviour of sodium deoxycholate in aqueous solution / Jover, A.; Fraga, F.; Meijide, F.; Vazquez Tato, J.; Cautela, J.; Del Giudice, A.; di Gregorio, M. C.. - In: JOURNAL OF COLLOID AND INTERFACE SCIENCE. - ISSN 0021-9797. - 604:(2021), pp. 415-428. [10.1016/j.jcis.2021.06.140]

Revealing the complex self-assembly behaviour of sodium deoxycholate in aqueous solution

Cautela J.;di Gregorio M. C.
2021

Abstract

Hypothesis: Sodium deoxycholate is a natural bile salt produced by animals and fulfilling important physiological processes. It is also used as dispersive surfactant and building block for self-assembled architectures in biology and material science. Although long debated, the study of its self-assembly in water is hereto incomplete and the models of the known aggregates are still controversial. This background suggests a complex scenario likely missing of additional mesophases. Experiments: Electron and optical microscopy techniques were crossed with SAXS data for the research. Findings: Novel rod, sponge, vesicle, lamellae, nanotube phases and reversible transitions among them arise at conditions (concentration, pH, temperature, ionic strength, ionic composition) fitting the physiological working environment of sodium deoxycholate. These findings enlarge the perspective towards different directions. The integration of the previous literature with this work removes any interpretative contradiction since all the structures cover the entire spectrum of phases expected for surfactants, thus being explained according to the Israelachvili's scheme. It is not trivial that a single molecule can show such a high structural variability. This fact highlights a very versatile system. Probably it is not a coincidence that it occurs in a multitasking biomolecule. These results furnish fundamental knowledge to clarify the bile salts’ role in vivo.
Bile salt; Biosurfactant; Lamellae; Mesophases transition; Nanotubes hydrogel; Self-assembly; Sponge structure; Vesicle; Scattering, Small Angle; Water; X-Ray Diffraction; Deoxycholic Acid; Surface-Active Agents
01 Pubblicazione su rivista::01a Articolo in rivista
Revealing the complex self-assembly behaviour of sodium deoxycholate in aqueous solution / Jover, A.; Fraga, F.; Meijide, F.; Vazquez Tato, J.; Cautela, J.; Del Giudice, A.; di Gregorio, M. C.. - In: JOURNAL OF COLLOID AND INTERFACE SCIENCE. - ISSN 0021-9797. - 604:(2021), pp. 415-428. [10.1016/j.jcis.2021.06.140]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1640620
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 7
social impact