Medulloblastoma (MB) is a childhood malignant brain tumour comprising four main subgroups characterized by different genetic alterations and rate of mortality. Among MB subgroups, patients with enhanced levels of the c-MYC oncogene (MBGroup3) have the poorest prognosis. Here we identify a previously unrecognized role of the pro-autophagy factor AMBRA1 in regulating MB. We demonstrate that AMBRA1 expression depends on c-MYC levels and correlates with Group 3 patient poor prognosis; also, knockdown of AMBRA1 reduces MB stem potential, growth and migration of MBGroup3 stem cells. At a molecular level, AMBRA1 mediates these effects by suppressing SOCS3, an inhibitor of STAT3 activation. Importantly, pharmacological inhibition of autophagy profoundly affects both stem and invasion potential of MBGroup3 stem cells, and a combined anti-autophagy and anti-STAT3 approach impacts the MBGroup3 outcome. Taken together, our data support the c-MYC/AMBRA1/STAT3 axis as a strong oncogenic signalling pathway with significance for both patient stratification strategies and targeted treatments of MBGroup3.

Targeting cancer stem cells in medulloblastoma by inhibiting AMBRA1 dual function in autophagy and STAT3 signalling / Nazio, Francesca; Po, Agnese; Abballe, Luana; Ballabio, Claudio; Diomedi Camassei, Francesca; Bordi, Matteo; Camera, Antonio; Caruso, Simona; Caruana, Ignazio; Pezzullo, Marco; Ferraina, Caterina; Milletti, Giacomo; Gianesello, Matteo; Reddel, Sofia; Dolores De Luca, Carmen; Ceglie, Donatella; Marinelli, Sara; Campello, Silvia; Papaleo, Elena; Miele, Evelina; Cacchione, Antonella; Carai, Andrea; Vinci, Maria; Velardi, Enrico; De Angelis, Biagio; Tiberi, Luca; Quintarelli, Concetta; Mastronuzzi, Angela; Ferretti, Elisabetta; Locatelli, Franco; Cecconi, Francesco. - In: ACTA NEUROPATHOLOGICA. - ISSN 0001-6322. - 142:3(2021), pp. 537-564. [10.1007/s00401-021-02347-7]

Targeting cancer stem cells in medulloblastoma by inhibiting AMBRA1 dual function in autophagy and STAT3 signalling

Agnese Po;Elisabetta Ferretti;Franco Locatelli;
2021

Abstract

Medulloblastoma (MB) is a childhood malignant brain tumour comprising four main subgroups characterized by different genetic alterations and rate of mortality. Among MB subgroups, patients with enhanced levels of the c-MYC oncogene (MBGroup3) have the poorest prognosis. Here we identify a previously unrecognized role of the pro-autophagy factor AMBRA1 in regulating MB. We demonstrate that AMBRA1 expression depends on c-MYC levels and correlates with Group 3 patient poor prognosis; also, knockdown of AMBRA1 reduces MB stem potential, growth and migration of MBGroup3 stem cells. At a molecular level, AMBRA1 mediates these effects by suppressing SOCS3, an inhibitor of STAT3 activation. Importantly, pharmacological inhibition of autophagy profoundly affects both stem and invasion potential of MBGroup3 stem cells, and a combined anti-autophagy and anti-STAT3 approach impacts the MBGroup3 outcome. Taken together, our data support the c-MYC/AMBRA1/STAT3 axis as a strong oncogenic signalling pathway with significance for both patient stratification strategies and targeted treatments of MBGroup3.
2021
medulloblastoma; ambra1; autophagy
01 Pubblicazione su rivista::01a Articolo in rivista
Targeting cancer stem cells in medulloblastoma by inhibiting AMBRA1 dual function in autophagy and STAT3 signalling / Nazio, Francesca; Po, Agnese; Abballe, Luana; Ballabio, Claudio; Diomedi Camassei, Francesca; Bordi, Matteo; Camera, Antonio; Caruso, Simona; Caruana, Ignazio; Pezzullo, Marco; Ferraina, Caterina; Milletti, Giacomo; Gianesello, Matteo; Reddel, Sofia; Dolores De Luca, Carmen; Ceglie, Donatella; Marinelli, Sara; Campello, Silvia; Papaleo, Elena; Miele, Evelina; Cacchione, Antonella; Carai, Andrea; Vinci, Maria; Velardi, Enrico; De Angelis, Biagio; Tiberi, Luca; Quintarelli, Concetta; Mastronuzzi, Angela; Ferretti, Elisabetta; Locatelli, Franco; Cecconi, Francesco. - In: ACTA NEUROPATHOLOGICA. - ISSN 0001-6322. - 142:3(2021), pp. 537-564. [10.1007/s00401-021-02347-7]
File allegati a questo prodotto
File Dimensione Formato  
Nazio_Medulloblastoma_2021pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 9.01 MB
Formato Unknown
9.01 MB Unknown

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1639208
Citazioni
  • ???jsp.display-item.citation.pmc??? 12
  • Scopus 25
  • ???jsp.display-item.citation.isi??? 21
social impact