Here, we describe a protocol to generate organoids from human thyroid cancer cells. Starting from the same patient-derived cells, we establish both organoids and primary lines. The organoid medium is supplemented with conditioned medium obtained from the primary cell line. This modification enables culture of the organoid lines for up to 10 months. Even after long-term culture, the organoids retain the genetic and phenotypic characteristics of their tissue of origin.

Establishment and maintenance of thyroid organoids from human cancer cells / Pecce, Valeria; Sponziello, Marialuisa; Bini, Simone; Grani, Giorgio; Durante, Cosimo; Verrienti, Antonella. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 3:2(2022). [10.1016/j.xpro.2022.101393]

Establishment and maintenance of thyroid organoids from human cancer cells

Pecce, Valeria
Co-primo
;
Sponziello, Marialuisa
Co-primo
;
Bini, Simone;Grani, Giorgio;Durante, Cosimo;Verrienti, Antonella
Ultimo
2022

Abstract

Here, we describe a protocol to generate organoids from human thyroid cancer cells. Starting from the same patient-derived cells, we establish both organoids and primary lines. The organoid medium is supplemented with conditioned medium obtained from the primary cell line. This modification enables culture of the organoid lines for up to 10 months. Even after long-term culture, the organoids retain the genetic and phenotypic characteristics of their tissue of origin.
2022
Cell Biology; Cancer; Organoids
01 Pubblicazione su rivista::01a Articolo in rivista
Establishment and maintenance of thyroid organoids from human cancer cells / Pecce, Valeria; Sponziello, Marialuisa; Bini, Simone; Grani, Giorgio; Durante, Cosimo; Verrienti, Antonella. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 3:2(2022). [10.1016/j.xpro.2022.101393]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1634127
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