Here, we describe a protocol to generate organoids from human thyroid cancer cells. Starting from the same patient-derived cells, we establish both organoids and primary lines. The organoid medium is supplemented with conditioned medium obtained from the primary cell line. This modification enables culture of the organoid lines for up to 10 months. Even after long-term culture, the organoids retain the genetic and phenotypic characteristics of their tissue of origin.
Establishment and maintenance of thyroid organoids from human cancer cells / Pecce, Valeria; Sponziello, Marialuisa; Bini, Simone; Grani, Giorgio; Durante, Cosimo; Verrienti, Antonella. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 3:2(2022). [10.1016/j.xpro.2022.101393]
Establishment and maintenance of thyroid organoids from human cancer cells
Pecce, Valeria
Co-primo
;Sponziello, MarialuisaCo-primo
;Bini, Simone;Grani, Giorgio;Durante, Cosimo;Verrienti, AntonellaUltimo
2022
Abstract
Here, we describe a protocol to generate organoids from human thyroid cancer cells. Starting from the same patient-derived cells, we establish both organoids and primary lines. The organoid medium is supplemented with conditioned medium obtained from the primary cell line. This modification enables culture of the organoid lines for up to 10 months. Even after long-term culture, the organoids retain the genetic and phenotypic characteristics of their tissue of origin.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
