The steps of the topoisomerization reaction by calf thymus DNA topoisomerase I on a DNA domain containing an intrinsically bent DNA sequence have been analyzed. High preferentiality of binding, cleavage and topoisomerization on the bent segment relative to the rest of the DNA domain was observed. These studies show the importance of the local DNA conformation in the reaction of eukaryotic DNA topoisomerase I and the interest of the use of this enzyme as a tool for the analysis of DNA conformation and DNA dynamics.
In vitro preferential topoisomerization of bent DNA / Caserta, M; Amadei, Andrea; DI MAURO, Ernesto; Camilloni, Giorgio. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 17:(1989), pp. 8463-8474. [10.1093/nar/17.21.8463]
In vitro preferential topoisomerization of bent DNA.
AMADEI, andrea;DI MAURO, Ernesto;CAMILLONI, Giorgio
1989
Abstract
The steps of the topoisomerization reaction by calf thymus DNA topoisomerase I on a DNA domain containing an intrinsically bent DNA sequence have been analyzed. High preferentiality of binding, cleavage and topoisomerization on the bent segment relative to the rest of the DNA domain was observed. These studies show the importance of the local DNA conformation in the reaction of eukaryotic DNA topoisomerase I and the interest of the use of this enzyme as a tool for the analysis of DNA conformation and DNA dynamics.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
