Fibrous dysplasia (FD) and hyperparathyroidism-jaw tumor syndrome (HPT-JT) are well-characterized benign bone fibro-osseous lesions. The intracellular mechanism leading to excessive deposition of fibrous tissue and alteration of differentiation processes leading to osteomalacia have not yet been fully clarified. Tissue Microarray (TMA)-based immunohistochemical expression of β-catenin, CK-AE1/AE3, Ki-67, cadherins and P-Runx2 were analyzed in archival samples from nine patients affected by FD and HPT-JT and in seven controls, with the aim of elucidating the contribution of these molecules (β-catenin, cadherins and P-Runx2) in the osteoblast differentiation pathway. β-catenin was strongly upregulated in FD, showing a hyper-cellulated pattern, while it was faintly expressed in bone tumors associated with HPT-JT. Furthermore, the loss of expression of OB-cadherin in osteoblast lineage in FD was accompanied by N-cadherin and P-cadherin upregulation (p < 0.05), while E-cadherin showed a minor role in these pathological processes. P-Runx2 showed over-expression in six out of eight cases of FD and stained moderately positive in the rimming lining osteoblasts in HPT-JT syndrome. β-catenin plays a central role in fibrous tissue proliferation and accompanies the lack of differentiation of osteoblast precursors in mature osteoblasts in FD. The study showed that the combined evaluation of the histological characteristics and the histochemical and immunohistochemical profile of key molecules involved in osteoblast differentiation are useful in the diagnosis, classification and therapeutic management of fibrous-osseous lesions.

Expression of beta-catenin, cadherins and P-Runx2 in fibro-osseous lesions of the jaw. Tissue microarray study / Pannone, G.; Nocini, R.; Santoro, A.; Spirito, F.; Nocini, P. F.; Papagerakis, S.; Franceschi, R. T.; Di Domenico, M.; Di Carlo, A.; Danelia, N.; Lo Muzio, L.. - In: BIOMOLECULES. - ISSN 2218-273X. - 12:4(2022). [10.3390/biom12040587]

Expression of beta-catenin, cadherins and P-Runx2 in fibro-osseous lesions of the jaw. Tissue microarray study

Di Carlo A.;
2022

Abstract

Fibrous dysplasia (FD) and hyperparathyroidism-jaw tumor syndrome (HPT-JT) are well-characterized benign bone fibro-osseous lesions. The intracellular mechanism leading to excessive deposition of fibrous tissue and alteration of differentiation processes leading to osteomalacia have not yet been fully clarified. Tissue Microarray (TMA)-based immunohistochemical expression of β-catenin, CK-AE1/AE3, Ki-67, cadherins and P-Runx2 were analyzed in archival samples from nine patients affected by FD and HPT-JT and in seven controls, with the aim of elucidating the contribution of these molecules (β-catenin, cadherins and P-Runx2) in the osteoblast differentiation pathway. β-catenin was strongly upregulated in FD, showing a hyper-cellulated pattern, while it was faintly expressed in bone tumors associated with HPT-JT. Furthermore, the loss of expression of OB-cadherin in osteoblast lineage in FD was accompanied by N-cadherin and P-cadherin upregulation (p < 0.05), while E-cadherin showed a minor role in these pathological processes. P-Runx2 showed over-expression in six out of eight cases of FD and stained moderately positive in the rimming lining osteoblasts in HPT-JT syndrome. β-catenin plays a central role in fibrous tissue proliferation and accompanies the lack of differentiation of osteoblast precursors in mature osteoblasts in FD. The study showed that the combined evaluation of the histological characteristics and the histochemical and immunohistochemical profile of key molecules involved in osteoblast differentiation are useful in the diagnosis, classification and therapeutic management of fibrous-osseous lesions.
2022
bone disease; cadherin; fibro-osseous lesions; fibrous dysplasia; HPT-JT syndrome; McCune–Albright; P-Runx2; tissue microarray; TMA; β-catenin; adenoma; cadherins; core binding factor alpha 1 subunit; fibroma; humans; beta catenin; hyperparathyroidism; jaw neoplasms
01 Pubblicazione su rivista::01a Articolo in rivista
Expression of beta-catenin, cadherins and P-Runx2 in fibro-osseous lesions of the jaw. Tissue microarray study / Pannone, G.; Nocini, R.; Santoro, A.; Spirito, F.; Nocini, P. F.; Papagerakis, S.; Franceschi, R. T.; Di Domenico, M.; Di Carlo, A.; Danelia, N.; Lo Muzio, L.. - In: BIOMOLECULES. - ISSN 2218-273X. - 12:4(2022). [10.3390/biom12040587]
File allegati a questo prodotto
File Dimensione Formato  
Pannone_Expression-beta-catenin_2022.pdf

accesso aperto

Note: https://www.mdpi.com/2218-273X/12/4/587
Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 4.88 MB
Formato Adobe PDF
4.88 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1630849
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 4
social impact