In the present communication, we report on the exploitation of a Bloch surface wave-enhanced fluorescence scheme for the detection of Interleukin (IL)-10 in a protein-rich buffer mimick-ing a biological sample. IL-10 is a cytokine known for its potent anti-inflammatory and immuno-suppressive effects. It is considered a valuable biomarker for prognostic prediction for both solid tumors and hematological malignancies, and recently, a distinguishing feature of hyperinflammation during severe viral infections. To demonstrate the validity of the technique, we transferred all the reagents and working concentrations used in a gold-standard technique, such as ELISA, to our assay, with a substantial reduction in the execution time and without using any enzymatic amplification during IL-10 recognition. We estimate a limit of detection (LoD) in terms of the concentration of IL-10 in solution of the order of 110 pg/mL (5.8 pM) with a 14% accuracy; in other terms, the presented technique is compatible with the assay range and resolution (1.6 pM) of commercial gold-standard ELISA kits. Moreover, such LoD successfully matches the concentrations reported in literature for IL-10 detection in COVID-19 patients, making the BSW-based sensors a viable solution for rapid and accurate screening of COVID-19-related molecules.

Enhanced fluorescence detection of interleukin 10 by means of 1d photonic crystals / Occhicone, A.; Del Porto, P.; Danz, N.; Munzert, P.; Sinibaldi, A.; Michelotti, F.. - In: CRYSTALS. - ISSN 2073-4352. - 11:12(2021). [10.3390/cryst11121517]

Enhanced fluorescence detection of interleukin 10 by means of 1d photonic crystals

Occhicone A.
Primo
;
Del Porto P.;Danz N.;Sinibaldi A.;Michelotti F.
Ultimo
2021

Abstract

In the present communication, we report on the exploitation of a Bloch surface wave-enhanced fluorescence scheme for the detection of Interleukin (IL)-10 in a protein-rich buffer mimick-ing a biological sample. IL-10 is a cytokine known for its potent anti-inflammatory and immuno-suppressive effects. It is considered a valuable biomarker for prognostic prediction for both solid tumors and hematological malignancies, and recently, a distinguishing feature of hyperinflammation during severe viral infections. To demonstrate the validity of the technique, we transferred all the reagents and working concentrations used in a gold-standard technique, such as ELISA, to our assay, with a substantial reduction in the execution time and without using any enzymatic amplification during IL-10 recognition. We estimate a limit of detection (LoD) in terms of the concentration of IL-10 in solution of the order of 110 pg/mL (5.8 pM) with a 14% accuracy; in other terms, the presented technique is compatible with the assay range and resolution (1.6 pM) of commercial gold-standard ELISA kits. Moreover, such LoD successfully matches the concentrations reported in literature for IL-10 detection in COVID-19 patients, making the BSW-based sensors a viable solution for rapid and accurate screening of COVID-19-related molecules.
Bloch surface waves; Fluorescence; Interleukin-10; One-dimensional photonic crystals; Sensors
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Enhanced fluorescence detection of interleukin 10 by means of 1d photonic crystals / Occhicone, A.; Del Porto, P.; Danz, N.; Munzert, P.; Sinibaldi, A.; Michelotti, F.. - In: CRYSTALS. - ISSN 2073-4352. - 11:12(2021). [10.3390/cryst11121517]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1625246
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