This work presents the realization of a label-free electrochemical immunosensor for the quick, cheap, and straightforward determination of atrazine. This biodevice is based on developing a technological platform where a gold screen printed electrode (Au-SPE) surface was modified by the electrodeposition of a highly porous gold layer. As an internal probe redox, a Prussian Blue thin layer (PB) was then electrosynthetized onto the modified Au-SPE. Atrazine antibody (Ab-ATZ) was immobilized using G protein-functionalized magnetic nanoparticles (MNPs@protG) to ensure the correct orientation of the antibody to enhance the immunoaffinity. Under optimum experimental conditions, the electrochemical characterization of the developed immunosensor displays a linearity range towards atrazine within 0.05–1.5 ng/mL, a LOD of 0.011 ng/mL good reproducibility and stability. The immunosensor was tested in the analysis of spiked drinking water samples with a mean recovery ranging from 95.7 to 108.4%. The overall good analytical performance of this immunodevice suggest its application for the screening and monitoring of atrazine in real matrices. Graphical Abstract: [Figure not available: see fulltext.]
Label-free magnetic nanoparticles-based electrochemical immunosensor for atrazine detection / Zumpano, Rosaceleste; Manghisi, Manuela; Polli, Francesca; D'Agostino, Cristine; Ietto, Federica; Favero, Gabriele; Mazzei, Franco. - In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. - ISSN 1618-2642. - 414:6(2022), pp. 2055-2064. [10.1007/s00216-021-03838-y]
Label-free magnetic nanoparticles-based electrochemical immunosensor for atrazine detection
Zumpano, Rosaceleste;Polli, Francesca;D'Agostino, Cristine;Favero, Gabriele;Mazzei, Franco
2022
Abstract
This work presents the realization of a label-free electrochemical immunosensor for the quick, cheap, and straightforward determination of atrazine. This biodevice is based on developing a technological platform where a gold screen printed electrode (Au-SPE) surface was modified by the electrodeposition of a highly porous gold layer. As an internal probe redox, a Prussian Blue thin layer (PB) was then electrosynthetized onto the modified Au-SPE. Atrazine antibody (Ab-ATZ) was immobilized using G protein-functionalized magnetic nanoparticles (MNPs@protG) to ensure the correct orientation of the antibody to enhance the immunoaffinity. Under optimum experimental conditions, the electrochemical characterization of the developed immunosensor displays a linearity range towards atrazine within 0.05–1.5 ng/mL, a LOD of 0.011 ng/mL good reproducibility and stability. The immunosensor was tested in the analysis of spiked drinking water samples with a mean recovery ranging from 95.7 to 108.4%. The overall good analytical performance of this immunodevice suggest its application for the screening and monitoring of atrazine in real matrices. Graphical Abstract: [Figure not available: see fulltext.]File | Dimensione | Formato | |
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