The heavy metal cadmium (Cd) affects root system development and quiescent center (QC)-definition in Arabidopsis root-apices. The brassinosteroids-(BRs)-mediated tolerance to heavy metals has been reported to occur by a modulation of nitric oxide (NO) and root auxin-localization. However, how BRs counteract Cd-action in different root types is unknown. This research aimed to find correlations between BRs and NO in response to Cd in Arabidopsis’s root system, monitor-ing their effects on QC-definition and auxin localization in root-apices. To this aim, root system developmental changes induced by low levels of 24-epibrassinolide (eBL) or by the BR-biosynthesis inhibitor brassinazole (Brz), combined or not with CdSO4, and/or with the NO-donor nitroprusside (SNP), were investigated using morpho-anatomical and NO-epifluorescence analyses, and moni-toring auxin-localization by the DR5::GUS system. Results show that eBL, alone or combined with Cd, enhances lateral (LR) and adventitious (AR) root formation and counteracts QC-disruption and auxin-delocalization caused by Cd in primary root/LR/AR apices. Exogenous NO enhances LR and AR formation in Cd-presence, without synergism with eBL. The NO-signal is positively affected by eBL, but not in Cd-presence, and BR-biosynthesis inhibition does not change the low NO-signal caused by Cd. Collectively, results show that BRs ameliorate Cd-effects on all root types acting independently from NO.

Brassinosteroids mitigate cadmium effects in Arabidopsis root system without any cooperation with nitric oxide / Della Rovere, Federica; Piacentini, Diego; Fattorini, Laura; Girardi, Nicoletta; Bellanima, Dario; Falasca, Giuseppina; Altamura, Maria Maddalena; Betti, Camilla. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 23:2(2022). [10.3390/ijms23020825]

Brassinosteroids mitigate cadmium effects in Arabidopsis root system without any cooperation with nitric oxide

Della Rovere, Federica;Piacentini, Diego;Fattorini, Laura;Girardi, Nicoletta;Falasca, Giuseppina;Altamura, Maria Maddalena;
2022

Abstract

The heavy metal cadmium (Cd) affects root system development and quiescent center (QC)-definition in Arabidopsis root-apices. The brassinosteroids-(BRs)-mediated tolerance to heavy metals has been reported to occur by a modulation of nitric oxide (NO) and root auxin-localization. However, how BRs counteract Cd-action in different root types is unknown. This research aimed to find correlations between BRs and NO in response to Cd in Arabidopsis’s root system, monitor-ing their effects on QC-definition and auxin localization in root-apices. To this aim, root system developmental changes induced by low levels of 24-epibrassinolide (eBL) or by the BR-biosynthesis inhibitor brassinazole (Brz), combined or not with CdSO4, and/or with the NO-donor nitroprusside (SNP), were investigated using morpho-anatomical and NO-epifluorescence analyses, and moni-toring auxin-localization by the DR5::GUS system. Results show that eBL, alone or combined with Cd, enhances lateral (LR) and adventitious (AR) root formation and counteracts QC-disruption and auxin-delocalization caused by Cd in primary root/LR/AR apices. Exogenous NO enhances LR and AR formation in Cd-presence, without synergism with eBL. The NO-signal is positively affected by eBL, but not in Cd-presence, and BR-biosynthesis inhibition does not change the low NO-signal caused by Cd. Collectively, results show that BRs ameliorate Cd-effects on all root types acting independently from NO.
adventitious roots; auxin localization; brassinazole; CdSO4; epibrassinolide; lateral roots; nitric oxide epifluorescence; nitroprusside; quiescent center; root apices
01 Pubblicazione su rivista::01a Articolo in rivista
Brassinosteroids mitigate cadmium effects in Arabidopsis root system without any cooperation with nitric oxide / Della Rovere, Federica; Piacentini, Diego; Fattorini, Laura; Girardi, Nicoletta; Bellanima, Dario; Falasca, Giuseppina; Altamura, Maria Maddalena; Betti, Camilla. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 23:2(2022). [10.3390/ijms23020825]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1615348
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