Background: Patients with primary antibody deficiencies are at risk in the current COVID-19 pandemic due to their impaired response to infection and vaccination. Specifically, patients with common variable immunodeficiency (CVID) generated poor spike-specific antibody and T cell responses after immunization. Methods: Thirty-four CVID convalescent patients after SARS-CoV-2 infection, 38 CVID patients immunized with two doses of the BNT162b2 vaccine, and 20 SARS-CoV-2 CVID convalescents later and immunized with BNT162b2 were analyzed for the anti-spike IgG production and the generation of spike-specific memory B cells and T cells. Results: Spike-specific IgG was induced more frequently after infection than after vaccination (82% vs. 34%). The antibody response was boosted in convalescents by vaccination. Although immunized patients generated atypical memory B cells possibly by extra-follicular or incomplete germinal center reactions, convalescents responded to infection by generating spike-specific memory B cells that were improved by the subsequent immunization. Poor spike-specific T cell responses were measured independently from the immunological challenge. Conclusions: SARS-CoV-2 infection primed a more efficient classical memory B cell response, whereas the BNT162b2 vaccine induced non-canonical B cell responses in CVID. Natural infection responses were boosted by subsequent immunization, suggesting the possibility to further stimulate the immune response by additional vaccine doses in CVID.

B Cell Response Induced by SARS-CoV-2 infection Is boosted by the BNT162b2 vaccine in primary antibody deficiencies / Pulvirenti, Federica; Fernandez Salinas, Ane; Milito, Cinzia; Terreri, Sara; Piano Mortari, Eva; Quintarelli, Concetta; Di Cecca, Stefano; Lagnese, Gianluca; Punziano, Alessandra; Guercio, Marika; Bonanni, Livia; Auria, Stefania; Villani, Francesca; Albano, Christian; Locatelli, Franco; Spadaro, Giuseppe; Carsetti, Rita; Quinti, Isabella. - In: CELLS. - ISSN 2073-4409. - 10:11(2021), pp. 1-15. [10.3390/cells10112915]

B Cell Response Induced by SARS-CoV-2 infection Is boosted by the BNT162b2 vaccine in primary antibody deficiencies

Milito, Cinzia;Piano Mortari, Eva;Guercio, Marika;Auria, Stefania;Villani, Francesca;Quinti, Isabella
2021

Abstract

Background: Patients with primary antibody deficiencies are at risk in the current COVID-19 pandemic due to their impaired response to infection and vaccination. Specifically, patients with common variable immunodeficiency (CVID) generated poor spike-specific antibody and T cell responses after immunization. Methods: Thirty-four CVID convalescent patients after SARS-CoV-2 infection, 38 CVID patients immunized with two doses of the BNT162b2 vaccine, and 20 SARS-CoV-2 CVID convalescents later and immunized with BNT162b2 were analyzed for the anti-spike IgG production and the generation of spike-specific memory B cells and T cells. Results: Spike-specific IgG was induced more frequently after infection than after vaccination (82% vs. 34%). The antibody response was boosted in convalescents by vaccination. Although immunized patients generated atypical memory B cells possibly by extra-follicular or incomplete germinal center reactions, convalescents responded to infection by generating spike-specific memory B cells that were improved by the subsequent immunization. Poor spike-specific T cell responses were measured independently from the immunological challenge. Conclusions: SARS-CoV-2 infection primed a more efficient classical memory B cell response, whereas the BNT162b2 vaccine induced non-canonical B cell responses in CVID. Natural infection responses were boosted by subsequent immunization, suggesting the possibility to further stimulate the immune response by additional vaccine doses in CVID.
2021
BNT162b2; COVID-1; SARS-CoV-2; antibody response; common variable immunodeficiencies; memory B cells; spike protein; third dose; vaccine; adult; antibodies, viral; BNT162 vaccine; COVID-19; convalescence; female; humans; immunization; immunoglobulin G; male; memory B cells; middle aged; primary immunodeficiency diseases; SARS-CoV-2; spike glycoprotein, coronavirus; T-lymphocytes
01 Pubblicazione su rivista::01a Articolo in rivista
B Cell Response Induced by SARS-CoV-2 infection Is boosted by the BNT162b2 vaccine in primary antibody deficiencies / Pulvirenti, Federica; Fernandez Salinas, Ane; Milito, Cinzia; Terreri, Sara; Piano Mortari, Eva; Quintarelli, Concetta; Di Cecca, Stefano; Lagnese, Gianluca; Punziano, Alessandra; Guercio, Marika; Bonanni, Livia; Auria, Stefania; Villani, Francesca; Albano, Christian; Locatelli, Franco; Spadaro, Giuseppe; Carsetti, Rita; Quinti, Isabella. - In: CELLS. - ISSN 2073-4409. - 10:11(2021), pp. 1-15. [10.3390/cells10112915]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1611954
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