3D microdosimetric model to plan and control in vitro drug delivery mediated by nsPEFs with GCPW system

Nanosecond pulsed electric fields (nsPEFs) with their wide frequency content, are recently known to be able to interact with cellular and subcellular membranes inducing reversible membrane permeabilization for biomedical applications. To improve treatments efficacy, lipid vesicles are studied and designed as smart delivery system able to respond to nsPEFs. In this work, authors report the study of an experimental bench suitable for in vitro exposure of cells and liposome nanocarriers to nsPEFs, compliant with the wideband requirements for nanosecond pulses. A multiphysics modelling able to predict cell and liposome nanoelectroporation is proposed to define the characteristics of signals to be used in experiments.