Implant-associated infections are characterized by microbial biofilm formation on implant surface, which renders the microbiological diagnosis challenging and requires, in the majority of cases, a complete device removal along with a prolonged antimicrobial therapy. Traditional cultures have shown unsatisfactory sensitivity and a significant advance in the field has been represented by both the application of the sonication technique for the detachment of live bacteria from biofilm and the implementation of metabolic and molecular assays. However, despite the recent progresses in the microbiological diagnosis have considerably reduced the rate of culture-negative infections, still their reported incidence is not negligible. Overall, several culture- and non-culture based methods have been developed for diagnosis optimization, which mostly relies on pre-operative and intra-operative (i.e., removed implants and surrounding tissues) samples. This review outlines the principal culture- and non-culture based methods for the diagnosis of the causative agents of implant-associated infections and gives an overview on their application in the clinical practice. Furthermore, advantages and disadvantages of each method are described.

Challenges in the Microbiological Diagnosis of Implant-Associated Infections: A Summary of the Current Knowledge / Oliva, A.; Miele, M. C.; Al Ismail, D.; Di Timoteo, F.; De Angelis, M.; Rosa, L.; Cutone, A.; Venditti, M.; Mascellino, M. T.; Valenti, P.; Mastroianni, C. M.. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - 12:(2021), pp. -1. [10.3389/fmicb.2021.750460]

Challenges in the Microbiological Diagnosis of Implant-Associated Infections: A Summary of the Current Knowledge

Oliva A.;Miele M. C.;Al Ismail D.;Di Timoteo F.;De Angelis M.;Rosa L.;Cutone A.;Venditti M.;Mascellino M. T.;Valenti P.;Mastroianni C. M.
2021

Abstract

Implant-associated infections are characterized by microbial biofilm formation on implant surface, which renders the microbiological diagnosis challenging and requires, in the majority of cases, a complete device removal along with a prolonged antimicrobial therapy. Traditional cultures have shown unsatisfactory sensitivity and a significant advance in the field has been represented by both the application of the sonication technique for the detachment of live bacteria from biofilm and the implementation of metabolic and molecular assays. However, despite the recent progresses in the microbiological diagnosis have considerably reduced the rate of culture-negative infections, still their reported incidence is not negligible. Overall, several culture- and non-culture based methods have been developed for diagnosis optimization, which mostly relies on pre-operative and intra-operative (i.e., removed implants and surrounding tissues) samples. This review outlines the principal culture- and non-culture based methods for the diagnosis of the causative agents of implant-associated infections and gives an overview on their application in the clinical practice. Furthermore, advantages and disadvantages of each method are described.
2021
biofilm; biotimer assay; culture-based methods; diagnosis; implant-associated infection; metabolic assays; molecular methods; sonication
01 Pubblicazione su rivista::01g Articolo di rassegna (Review)
Challenges in the Microbiological Diagnosis of Implant-Associated Infections: A Summary of the Current Knowledge / Oliva, A.; Miele, M. C.; Al Ismail, D.; Di Timoteo, F.; De Angelis, M.; Rosa, L.; Cutone, A.; Venditti, M.; Mascellino, M. T.; Valenti, P.; Mastroianni, C. M.. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - 12:(2021), pp. -1. [10.3389/fmicb.2021.750460]
File allegati a questo prodotto
File Dimensione Formato  
Oliva _Challenges_2021.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 1.4 MB
Formato Adobe PDF
1.4 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1591095
Citazioni
  • ???jsp.display-item.citation.pmc??? 12
  • Scopus 17
  • ???jsp.display-item.citation.isi??? 16
social impact