Preparación de agregados enzimáticos de Lacasas y su aplicación en el tratamiento de colorantes textiles

It is well known that textile effluents have a dreadful impact on the environment. The biological treatment of these effluents has been performed by using extracellular enzymes from ligninolytic fungi immobilized without support systems. The objective of the present project was to evaluate the optimum conditions for the immobilization of laccase enzymes from Trametes sp. in enzymatic aggregates known as CLEAs (cross-linked Enzymatic Aggregates). The laccase activity was colorimetrically determined by using ABTS and DMP as substrates. In addition, the CLEAs’ decolorization activity was spectrophotometrically evaluated. The results showed that the greater laccase activity of CLEAs was obtained by using isopropanol and 25 mM glutaraldehyde as precipitating agents. Furthermore, CLEAs were able to decolorize different textile dyes even during successive cycles. The present study demonstrates that CLEAs with laccase activity could serve as catalysts agents for the treatment of textile dyes.