We have observed that treatment of human glioma cells with morphine in the nanomolar range of concentration affects the mitochondrial membrane potential. The effect is specific to morphine and is mediated by naloxone-sensitive receptors, and is thus better observed on glioma cells treated with desipramine; moreover, the mitochondrial impairment is not inducible by fentanyl or methadone treatment and is prevented by the nitric oxide (NO) synthase inhibitor L-NAME. We conclude that in cultured glioma cells, the morphine-induced NO release decreases the mitochondrial membrane potential, as one might expect based on the rapid inhibition of the respiratory chain by NO. The identification of new intra-cellular pathways involved in the mechanism of action of morphine opens additional hypotheses, providing a novel rationale relevant to the therapy and toxicology of opioids

Morphine but not fentanyl and methadone affects mitochondrial membrane potential by inducing NO release in glioma cells / Mastronicola, Daniela; Arcuri, E.; Arese, Marzia; Bacchi, Antonella; Mercadante, S.; Cardelli, P.; Citro, G.; Sarti, Paolo. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - 61:(2004), pp. 2991-2997. [10.1007/s00018-004-4371-x]

Morphine but not fentanyl and methadone affects mitochondrial membrane potential by inducing NO release in glioma cells.

MASTRONICOLA, Daniela;ARESE, Marzia;BACCHI, Antonella;SARTI, Paolo
2004

Abstract

We have observed that treatment of human glioma cells with morphine in the nanomolar range of concentration affects the mitochondrial membrane potential. The effect is specific to morphine and is mediated by naloxone-sensitive receptors, and is thus better observed on glioma cells treated with desipramine; moreover, the mitochondrial impairment is not inducible by fentanyl or methadone treatment and is prevented by the nitric oxide (NO) synthase inhibitor L-NAME. We conclude that in cultured glioma cells, the morphine-induced NO release decreases the mitochondrial membrane potential, as one might expect based on the rapid inhibition of the respiratory chain by NO. The identification of new intra-cellular pathways involved in the mechanism of action of morphine opens additional hypotheses, providing a novel rationale relevant to the therapy and toxicology of opioids
2004
01 Pubblicazione su rivista::01a Articolo in rivista
Morphine but not fentanyl and methadone affects mitochondrial membrane potential by inducing NO release in glioma cells / Mastronicola, Daniela; Arcuri, E.; Arese, Marzia; Bacchi, Antonella; Mercadante, S.; Cardelli, P.; Citro, G.; Sarti, Paolo. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - 61:(2004), pp. 2991-2997. [10.1007/s00018-004-4371-x]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/15830
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 9
  • Scopus 30
  • ???jsp.display-item.citation.isi??? 27
social impact