Adult stem cells are nowadays used for treating several pathologies. A putative stem cell population was found in the adipose tissue of mammals and canine adipose tissue-derived-mesenchymal stem cells (cA-MSC) have been shown to possess the capacity to differentiate into several lineages. The main goal of our research was to fully characterize cA-MSC and examine the effects of cryopreservation on their stemness features. Each sample of cA-MSC was analyzed immediately and then again after being frozen in liquid nitrogen for one year. After the cryopreservation period cells conserved their fibroblast-like morphology, alkaline phosphatase positivity and CD expression but showed a lower proliferation ratio and a lower telomerase activity in comparison with fresh cells. Finally, the cryopreservation protocol did not change the cA-MSC adipogenic, osteogenic and myogenic differentiative potential. Our data demonstrate that stored cA-MSC might represent a promising type of progenitor cell for autologous cellular-based therapies in veterinary medicine.
Canine adipose-derived-mesenchymal stem cells do not lose stem features after a long-term cryopreservation / Martinello, T; Bronzini, I; Maccatrozzo, L; Mollo, A; Sampaolesi, Maurilio; Mascarello, F; Decaminada, M; Patruno, M.. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - 91:1(2011), pp. 18-24. [10.1016/j.rvsc.2010.07.024]