Satellite cells that reside on the myofibre surface are crucial for the muscle homeostasis and regeneration. Aging goes along with a less effective regeneration of skeletal muscle tissue mainly due to the decreased myogenic capability of satellite cells. This phenomenon impedes proper maintenance and contributes to the age-associated decline in muscle mass, known as sarcopenia. The myogenic potential impairment does not depend on a reduced myogenic cell number, but mainly on their difficulty to complete a differentiation program. The unbalanced production of reactive oxygen species in elderly people could be responsible for skeletal muscle impairments. microRNAs are conserved post-transcriptional regulators implicated in numerous biological processes including adult myogenesis. Here, we measure the ROS level and analyze myomiR (miR-1, miR-133b and miR-206) expression in human myogenic precursors obtained from Vastus lateralis of elderly and young subjects to provide the molecular signature responsible for the differentiation impairment of elderly activated satellite cells.

Myomir dysregulation and reactive oxygen species in aged human satellite cells / Di Filippo, Ester Sara; Mancinelli, Rosa; Pietrangelo, Tiziana; La Rovere, Rita Maria Laura; Quattrocelli, Mattia; Sampaolesi, Maurilio; Fulle, Stefania. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 473:2(2016), pp. 462-470. [10.1016/j.bbrc.2016.03.030]

Myomir dysregulation and reactive oxygen species in aged human satellite cells

SAMPAOLESI, MAURILIO;
2016

Abstract

Satellite cells that reside on the myofibre surface are crucial for the muscle homeostasis and regeneration. Aging goes along with a less effective regeneration of skeletal muscle tissue mainly due to the decreased myogenic capability of satellite cells. This phenomenon impedes proper maintenance and contributes to the age-associated decline in muscle mass, known as sarcopenia. The myogenic potential impairment does not depend on a reduced myogenic cell number, but mainly on their difficulty to complete a differentiation program. The unbalanced production of reactive oxygen species in elderly people could be responsible for skeletal muscle impairments. microRNAs are conserved post-transcriptional regulators implicated in numerous biological processes including adult myogenesis. Here, we measure the ROS level and analyze myomiR (miR-1, miR-133b and miR-206) expression in human myogenic precursors obtained from Vastus lateralis of elderly and young subjects to provide the molecular signature responsible for the differentiation impairment of elderly activated satellite cells.
2016
aging; human satellite cells; myomiRNAs; ROS; sarcopenia; adolescent; adult; aged; aged; 80 and over; cells; cultured; humans; male; microRNAs; reactive oxygen species; sarcopenia; satellite cells; skeletal muscle; young adult; aging; gene expression regulation; muscle development; biophysics; biochemistry; molecular biology; cell biology
01 Pubblicazione su rivista::01a Articolo in rivista
Myomir dysregulation and reactive oxygen species in aged human satellite cells / Di Filippo, Ester Sara; Mancinelli, Rosa; Pietrangelo, Tiziana; La Rovere, Rita Maria Laura; Quattrocelli, Mattia; Sampaolesi, Maurilio; Fulle, Stefania. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 473:2(2016), pp. 462-470. [10.1016/j.bbrc.2016.03.030]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1581839
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