Met-Activating Genetically Improved Chimeric Factor-1 is an engineered protein that contains two human Met-binding domains that protects myogenic precursors against apoptosis and increases their fusion ability enhancing muscle differentiation. Previous experiments in both homozygous and hemizygous transgenic mice demonstrated that the skeletal muscle specific expression of Magic-F1 can induce a constitutive muscular hypertrophy, increasing the vessel number in fast twitch fibers, also improving running performance and accelerating muscle regeneration after injury [1]. We also examined the temporal and spatial expression pattern of Magic-F1 in developing organs and tissues of mesenchymal origin. We found that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells [2]. In order to evaluate the therapeutic potential of Magic-F1, we tested its effect on multipotent and pluripotent stem cells [3]. Murine mesoangioblasts (adult vessel-associated stem cells) expressing Magic-F1 were able to differentiate spontaneously forming myotubes. In addition, in Magic-F1 inducible murine embryonic stem cells subjected to myogenic differentiation, the presence of recombinant protein resulted in improved myogenic commitment. Finally, the microarray analysis of Magic-F1+/+ satellite cells evidenced transcriptomic changes in genes involved in the control of muscle growth, development and vascularisation [4]. Taken together our results candidate Magic-F1 as a potent myogenic inducer, able to affect positively the vascular network, increasing vessel number in fast twitch fibers and modulating the gene expression profile in myogenic progenitors

Muscle hypertrophy and vascularization induction using recombinant proteins / Ronzoni, Fl; Ceccarelli, G; Benedetti, L; Bellazzi, R; Cusella De Angelis, Mg; Sampaolesi, M.. - In: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY. - ISSN 2038-5129. - 122:1 supplement(2017), pp. 185-185. ((Intervento presentato al convegno 71th Meeting of the Italian Society of Anatomy and Histology tenutosi a Taormina.

Muscle hypertrophy and vascularization induction using recombinant proteins

Bellazzi R;Sampaolesi M.
2017

Abstract

Met-Activating Genetically Improved Chimeric Factor-1 is an engineered protein that contains two human Met-binding domains that protects myogenic precursors against apoptosis and increases their fusion ability enhancing muscle differentiation. Previous experiments in both homozygous and hemizygous transgenic mice demonstrated that the skeletal muscle specific expression of Magic-F1 can induce a constitutive muscular hypertrophy, increasing the vessel number in fast twitch fibers, also improving running performance and accelerating muscle regeneration after injury [1]. We also examined the temporal and spatial expression pattern of Magic-F1 in developing organs and tissues of mesenchymal origin. We found that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells [2]. In order to evaluate the therapeutic potential of Magic-F1, we tested its effect on multipotent and pluripotent stem cells [3]. Murine mesoangioblasts (adult vessel-associated stem cells) expressing Magic-F1 were able to differentiate spontaneously forming myotubes. In addition, in Magic-F1 inducible murine embryonic stem cells subjected to myogenic differentiation, the presence of recombinant protein resulted in improved myogenic commitment. Finally, the microarray analysis of Magic-F1+/+ satellite cells evidenced transcriptomic changes in genes involved in the control of muscle growth, development and vascularisation [4]. Taken together our results candidate Magic-F1 as a potent myogenic inducer, able to affect positively the vascular network, increasing vessel number in fast twitch fibers and modulating the gene expression profile in myogenic progenitors
File allegati a questo prodotto
File Dimensione Formato  
Ronzoni_Muscle_2017.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 155.16 kB
Formato Adobe PDF
155.16 kB Adobe PDF Visualizza/Apri PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1581735
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? ND
social impact