Beam self-cleaning (BSC) in graded-index (GRIN) multimode fibers (MMFs) has been recently reported by different research groups. Driven by the interplay between Kerr effect and beam self-imaging, BSC counteracts random mode coupling, and forces laser beams to recover a quasi-single mode profile at the output of GRIN fibers. Here we show that the associated self-induced spatiotemporal reshaping allows for improving the performances of nonlinear fluorescence (NF) microscopy and endoscopy using multimode optical fibers. We experimentally demonstrate that the beam brightness increase, induced by self-cleaning, enables two and three-photon imaging of biological samples with high spatial resolution. Temporal pulse shortening accompanying spatial beam clean-up enhances the output peak power, hence the efficiency of nonlinear imaging. We also show that spatiotemporal supercontinuum (SC) generation is well-suited for large-band NF imaging in visible and infrared domains. We substantiated our findings by multiphoton fluorescence imaging in both microscopy and endoscopy configurations.

Spatiotemporal beam self-cleaning for high-resolution nonlinear fluorescence imaging with multimode fiber / Moussa, N. O.; Mansuryan, T.; Hage, C. -H.; Fabert, M.; Krupa, K.; Tonello, A.; Ferraro, M.; Leggio, L.; Zitelli, M.; Mangini, F.; Niang, A.; Millot, G.; Papi, M.; Wabnitz, S.; Couderc, V.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 11:1(2021). [10.1038/s41598-021-96753-2]

Spatiotemporal beam self-cleaning for high-resolution nonlinear fluorescence imaging with multimode fiber

Ferraro M.;Leggio L.;Zitelli M.;Mangini F.;Niang A.;Papi M.;Wabnitz S.;
2021

Abstract

Beam self-cleaning (BSC) in graded-index (GRIN) multimode fibers (MMFs) has been recently reported by different research groups. Driven by the interplay between Kerr effect and beam self-imaging, BSC counteracts random mode coupling, and forces laser beams to recover a quasi-single mode profile at the output of GRIN fibers. Here we show that the associated self-induced spatiotemporal reshaping allows for improving the performances of nonlinear fluorescence (NF) microscopy and endoscopy using multimode optical fibers. We experimentally demonstrate that the beam brightness increase, induced by self-cleaning, enables two and three-photon imaging of biological samples with high spatial resolution. Temporal pulse shortening accompanying spatial beam clean-up enhances the output peak power, hence the efficiency of nonlinear imaging. We also show that spatiotemporal supercontinuum (SC) generation is well-suited for large-band NF imaging in visible and infrared domains. We substantiated our findings by multiphoton fluorescence imaging in both microscopy and endoscopy configurations.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1578793
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