The use of psychoactive substances is a serious problem in today’s society and reliable methods of analysis are necessary to confirm their occurrence in biological matrices. In this work, a green sample preparation technique prior to HPLC-MS analysis was successfully applied to the extraction of 14 illicit drugs from urine samples. The isolation procedure was a dispersive liquid– liquid microextraction based on the use of a low transition temperature mixture (LTTM), composed of choline chloride and sesamol in a molar ratio 1:3 as the extracting solvent. This mixture was classified as LTTM after a thorough investigation carried out by FTIR and DSC, which recorded a glass transition temperature at −71◦C. The extraction procedure was optimized and validated according to the main Food and Drug Administration (FDA) guidelines for bioanalytical methods, obtaining good figures of merit for all parameters: the estimated lower limit of quantitation (LLOQ) values were between 0.01 µg L−1 (bk-MMBDB) and 0.37 µg L−1 (PMA); recoveries, evaluated at very low spike levels (in the ng-µg L−1 range), spanned from 55% (MBDB) to 100% (bk-MMBDB and MDPV); finally, both within-run and between-run precisions were lower than 20% (LLOQ) and 15% (10xLLOQ).

Application of a low transition temperature mixture for the dispersive liquid–liquid microextraction of illicit drugs from urine samples / Gallo, Valeria; Tomai, Pierpaolo; Di Lisio, Valerio; Dal Bosco, Chiara; D'Angelo, Paola; Fanali, Chiara; D'Orazio, Giovanni; Silvestro, Ilaria; Pico, Yolanda; Gentili, Alessandra. - In: MOLECULES. - ISSN 1420-3049. - 26:17(2021). [10.3390/molecules26175222]

Application of a low transition temperature mixture for the dispersive liquid–liquid microextraction of illicit drugs from urine samples

Gallo, Valeria;Tomai, Pierpaolo;Di Lisio, Valerio;Dal Bosco, Chiara;D'angelo, Paola;D'orazio, Giovanni;Silvestro, Ilaria;Gentili, Alessandra
2021

Abstract

The use of psychoactive substances is a serious problem in today’s society and reliable methods of analysis are necessary to confirm their occurrence in biological matrices. In this work, a green sample preparation technique prior to HPLC-MS analysis was successfully applied to the extraction of 14 illicit drugs from urine samples. The isolation procedure was a dispersive liquid– liquid microextraction based on the use of a low transition temperature mixture (LTTM), composed of choline chloride and sesamol in a molar ratio 1:3 as the extracting solvent. This mixture was classified as LTTM after a thorough investigation carried out by FTIR and DSC, which recorded a glass transition temperature at −71◦C. The extraction procedure was optimized and validated according to the main Food and Drug Administration (FDA) guidelines for bioanalytical methods, obtaining good figures of merit for all parameters: the estimated lower limit of quantitation (LLOQ) values were between 0.01 µg L−1 (bk-MMBDB) and 0.37 µg L−1 (PMA); recoveries, evaluated at very low spike levels (in the ng-µg L−1 range), spanned from 55% (MBDB) to 100% (bk-MMBDB and MDPV); finally, both within-run and between-run precisions were lower than 20% (LLOQ) and 15% (10xLLOQ).
2021
low transition temperature mixtures; deep eutectic solvents; dispersive liquid–liquid microextraction; drugs; high performance liquid chromatography; illicit drugs; urine; biological samples
01 Pubblicazione su rivista::01a Articolo in rivista
Application of a low transition temperature mixture for the dispersive liquid–liquid microextraction of illicit drugs from urine samples / Gallo, Valeria; Tomai, Pierpaolo; Di Lisio, Valerio; Dal Bosco, Chiara; D'Angelo, Paola; Fanali, Chiara; D'Orazio, Giovanni; Silvestro, Ilaria; Pico, Yolanda; Gentili, Alessandra. - In: MOLECULES. - ISSN 1420-3049. - 26:17(2021). [10.3390/molecules26175222]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1576500
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